玉米茎尖导入SAG_(12)基因的初步研究  

Transformation of SAG_(12) Gene into Apical Point of Maize Plant

作　　者：曲文利[1] 孙传波[1] 孟凡梅[1] 陶蕊[1] 郭佳[1] 李海华[1] 张举仁[2] 郝东云[1] 袁英[1] 

机构地区：[1]吉林省农业科学院生物技术研究中心,长春130033 [2]山东大学生命科学学院,济南250100

出　　处：《吉林农业科学》2012年第4期10-11,29,共3页Journal of　Jilin Agricultural Sciences

基　　金：吉林省科技发展项目(20076010)

摘　　要：选用玉米优良自交系郑58的茎尖为受体,研究以玉米茎尖为受体进行农杆菌转化体系的可行性,用农杆菌介导法将耐盐碱基因SAG12转入玉米中,获得110株转化苗,经过300 mg/L的除草剂(Basta)筛选,共获得21株转基因植株。进一步进行PCR检测,其中14株表现阳性,转化率达12.73%。初步证明外源基因已经整合到玉米基因组中,以玉米茎尖作为受体孢子体的转化系统可用于基因转化。The SAG12 gene was transferred into the apical point of maize plant of inbred line ’Zheng 58’ by Agro-bacterium tumefaciens mediated transformation to study the feasibility of this system.21 transgenic plants were obtained after selecting with 300mg/L Basta and 14 of them were confirmed positive by PCR amplification.The frequency of transformation reached about 12.73%.The result showed that the foreign gene has integrated into maize genome and the apical point of maize plant can be used as receipt sporophyte for transformation.

关 键 词：玉米 茎尖 农杆菌介导 SAG12基因 

分 类 号：S513[农业科学—作物学]