贵州白香猪γ干扰素基因的克隆与序列分析  

Cloning and Sequence Analysis of Guizhou Baixiang Pig Interferon Gamma Gene

作　　者：曾智勇[1,2] 周莉[1] 刘志杰[1] 梁海英[3] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州省动物疫病研究室,贵州贵阳550025 [3]贵州大学教学实验场,贵州贵阳550025

出　　处：《贵州农业科学》2012年第8期151-153,共3页Guizhou Agricultural Sciences

基　　金：贵州省科学技术基金项目"猪伪狂犬病病毒贵州株的分离鉴定及gE基因的克隆研究"[黔科合J字(2007)2065];贵州省农业科技攻关项目"贵州省规模化猪场繁殖障碍性疫病的调查及综合防治模式的研究"[黔科合NY字(2010)3042]

摘　　要：为获得贵州白香猪γ干扰素基因,以提取的经刀豆蛋白A诱导培养的贵州白香猪外周血淋巴细胞总RNA为模板,扩增全长猪γ干扰素基因,并克隆至pMD18-T Simple载体后测序分析。结果表明,pIFN-γ基因全长501bp,可编码166个氨基酸,其中前23位氨基酸为信号肽序列;pIFN-γ含有2个潜在N-糖基化位点;贵州白香猪与国内地方猪种之间pIFN-γ基因差异不大,具有很近的亲缘关系,其中与藏猪、成华猪、枫径猪、梅山猪的同源性达100%,而与内江猪的亲缘关系相对较远,与荣昌猪(DQ902588)亲缘最远。In order to obtain the pIFN-gamma gene,the total RNA was extracted from peripheral blood mononuclear cells（PBMC）,which was isolated from Guizhou Baixiang Pig and induced with concanavaline A（ConA）.The porcine interferon gamma gene was amplified by RT-PCR from the total RNA,and cloned to the pMD18-T simple vector for sequencing.The results of sequence analysis indicated that the amplified pIFN-gamma was 501 bp,coding 166 amino acids,which included two potential N-glycosylation site,and 1～23 amino acid sequence for the signal peptide.There was smaller difference between the Guizhou Baixiang Pig and local domestic pigs for pIFN-gamma gene,with a close genetic relationship.Guizhou baixiang pig had 100 percent amino acid homology with the Tibet pig,Chenghua pig,Fengjing pig and Meishan pig,but with Neijiang pig genetic relationship was relatively distant,the farthest relatives with Rongchang pig（DQ902588）.

关 键 词：贵州白香猪 干扰素-Γ 基因克隆 序列分析 

分 类 号：S828.212[农业科学—畜牧学]