褪黑素与盐酸多巴胺在猪精液冷冻保存中的应用效果  

Effects of Melatonin and Dopamine Hydrochloride on Cryopreservation of Boar Spermatozoa

作　　者：杨宝卫[1] 李美珍 黄明光 胡传水[4] 黄卫红 胡传活[1] 

机构地区：[1]广西大学动物科技学院,广西南宁530005 [2]广西畜禽品种改良站,广西南宁530001 [3]广西科达畜禽改良有限责任公司,广西南宁530001 [4]广西农业职业技术学院,广西南宁530007

出　　处：《贵州农业科学》2012年第8期154-155,160,共3页Guizhou Agricultural Sciences

基　　金：广西教育厅科研项目"液态或冷冻保存猪精子凋亡率的变化及应对措施研究"(201012MS001);南宁市科技开发计划项目"抗凋亡理论在猪精液常温或冷冻保存前后品质改良中的应用研究"(201107016C)

摘　　要：为给改良猪精液冷冻稀释液配方提供参考数据,分析了不同浓度褪黑素和盐酸多巴胺对猪精子冷冻后畸形率和顶体完整率的影响。结果表明,10mmol/L褪黑素及3种浓度的盐酸多巴胺(0.1mmol/L,1mmol/L,10mmol/L)均能有效改善冻后猪精子的畸形率和顶体完整率,两种试剂均可在猪精液冷冻中进一步推广应用。To provide reference data for the improvement of the composition of the diluent for cryopreservation of boar spermatozoa,the effects of different concentrations of melatonin and dopamine hydrochloride on abnormality,acrosomal integrity of frozen-thawed boar spermatozoa were analyzed.The results showed that 10 mmol/L melatonin or three doses of dopamine hydrochloride（0.1 mmol/L,1 mmol/L,10 mmol/L） could significantly improve abnormality,acrosomal integrity of frozen-thawed boar spermatozoa.It indicated that both of the two drugs deserved further extension and utilization to boar semen cryopreservation.

关 键 词：褪黑素 盐酸多巴胺 猪 精液 冷冻保存 

分 类 号：S828.141[农业科学—畜牧学]