内蒙古蚋类rDNA-ITS1序列分析  

Sequence Analysis of rDNA-ITS1 of Black Flies from Inner Mongolia

作　　者：徐旭[1] 杨明[1] 陈汉彬[1] 

机构地区：[1]贵阳医学院,贵阳550004

出　　处：《贵州科学》2012年第4期25-28,共4页Guizhou Science

基　　金：国家自然科学基金[30660021]

摘　　要：对内蒙古2属4亚属7蚋种rDNA-ITS1长度及序列变异进行分析。陆氏后克蚋和长鞭纺蚋的ITS1长340nt左右,宽跗副布蚋、辽宁蚋、内蒙蚋、纳克蚋和红头厌蚋的ITS1长约100nt。陆氏后克蚋的ITS1长度,可作为将后克蚋属归为蚋族的一个佐证。宽跗副布蚋的ITS1序列与近来LaRue等提出的蚋类ITS1存在由8个短保守区构成的39nt核心的这一结论仅部分相符,提示该结论的适用范围有一定限制。A sample of 7 species from Inner Mongolia spread over two genera four subgenera is used to perform a length and sequence variability study of the internal transcribed spacer 1 （ ITS1 ） of rDNA. ITS1 of Metacnephia lui and Simulium （Neverrnannia） longiflageUum have a length of about 340 nt, those orS. （Boophthora） erythroceph- ala, S. （ Parabyssodon） transiens, S. （ S. ） leimonses, S. （ S. ） liaoningense and S. （ S. ） nacojapi about 100 nt. The ITS1 length of M. lui can be evidence to support the opinion that genus Metacnephia belongs to tribe Simuliini. Sequence of ITS1 of S. （P.） transiens is partially corresponded with a conclusion about core sequence in ITS1 of black fly proposed in 2009 by LaRue et al. who derived that there existed a 39 nt common core composed of eight short conserve blocks. This indicates that LaRue＇ s generalization has a limited application to Simuliidae itself.

关 键 词：蚋科 ITS1 序列变异 内蒙古 

分 类 号：Q969.91[生物学—昆虫学]