壁虎粗多肽诱导人肝癌细胞HepG2凋亡的机制研究  

Apoptotic Mechanism of Gecko Crude Peptides on Human Liver Carcinoma Cell Line HepG2

作　　者：宋莹[1] 王建刚[1] 崔朝初[1] 钱昕[1] 李瑞芳[1] 段冷昕[1] 刘玲[1] 席守民[1] 

机构地区：[1]河南科技大学医学院药理学与医学分子生物学重点实验室,河南洛阳471003

出　　处：《中药材》2012年第6期863-866,共4页Journal of Chinese Medicinal Materials

基　　金：河南省医药卫生重点项目(200903109);河南省科技重点攻关项目(102102310063)

摘　　要：目的:体外实验研究壁虎粗多肽(Gecko Crude Peptides,GCPS)诱导人肝癌细胞HepG2凋亡的机制。方法:采用MTT法检测GCPS对肝癌细胞HepG2增殖的影响;采用Hoechst33258荧光染色观察细胞凋亡的形态学改变,western-blot检测BAX和BCL-2蛋白的表达,观察壁虎粗多肽对人肝癌细胞凋亡的影响。结果:GCPS可以抑制肝癌细胞HepG2增殖,作用呈浓度和时间依赖性,其IC50为1.2 mg/mL;在GCPS的作用下,HepG2呈凋亡形态学改变,且GCPS(1.6 mg/mL、0.8 mg/mL)可以降低bcl-2的表达,升高蛋白bax的表达。结论:GCPS对HepG2细胞有增殖抑制作用,诱导其凋亡是其作用机理之一。Objective ： To investigate the effect of Gecko crude peptides （GCPS） on human liver carcinoma HepG2 cells and its mechanism. Methods：MTT assay was used to analyze the effect of the GCPS on the proliferation of HepG2 Cell;Nucleus change of HepG2 treated with GCP was observed by Hoechst33258 fluorescence staining, and BAX and BCL-2 were detected with western-blot assay. Results： GCPS could inhibit the proliferation of HepG2 Cell in a time and dosage dependent way, and its half-maximal inhibitory concentration （IC50） was 1.2 mg/mL; HepG2 pretreated with GCPS showed apoptotic morphological changes. GCPS（ 1.6 mg/mL,0. 8 mg/mL） could decrease the expression of BCL-2 protein, and increase the expression of BAX protein. Conclusion：GCPS can inhibit the proliferation of HepG2 cell. The mechanism may be related to the induction apoptosis of HepG2.

关 键 词：壁虎粗多肽 HEPG2 BAX bcl-2 凋亡 

分 类 号：R285.5[医药卫生—中药学]