比较同一复方中用红芪与用黄芪对免疫抑制小鼠体液免疫的作用  

Comparative Study of Hedysari Radix and Astragali Radix Alternative Classic Tonification Prescriptions on Humoral Immunity in Immunosuppressed Mice

作　　者：卫东锋[1] 张李峰[2] 程卫东[1,3] 桂曼曼[1] 李雪嫣[1] 韦艳霞[1] 鲍英存[1] 

机构地区：[1]兰州大学中西医结合研究所,甘肃兰州730000 [2]兰州大学基础医学院免疫学研究所,甘肃兰州730000 [3]北京师范大学,北京100009

出　　处：《中药材》2012年第6期944-948,共5页Journal of Chinese Medicinal Materials

基　　金：中国博士后科学基金(201003073);中国博士后科学基金(20090450017);教育部高等学校博士点基金(200807330006)

摘　　要：目的:比较红芪替换经典复方中的黄芪后对免疫抑制小鼠体液免疫的调节作用。方法:通过给小鼠注射环磷酰胺建立免疫抑制小鼠模型,以相同剂量的红芪替换补中益气口服液、益气养血口服液、复芪止汗颗粒和玉屏风口服液中的黄芪后灌胃进行体内拮抗实验,测定小鼠脾脏指数、血清HC50、CD19+B淋巴细胞亚群以及血清IL-4含量。结果:环磷酰胺免疫抑制小鼠经各黄芪复方和红芪复方作用后小鼠脾脏指数、血清HC50、CD19+B淋巴细胞亚群及血清IL-4含量均出现不同程度的增加;其中含红芪的玉屏风水提物在提高环磷酰胺免疫抑制小鼠脾脏指数方面优于含黄芪的玉屏风口服液,其余各复方经比较虽然红芪复方作用稍强于黄芪复方,但差异无统计学。结论:红芪替换黄芪配伍其他药物后与原方相比在调节体液免疫方面具有相似作用。Objective：To compare the regulating effects of Hedysari Radix and Astragali Radix alternative classic tonification pre- scriptions on humoral immunity in immunosuppressed mice. Methods：The immunosuppressed mouse model was induced by cyclophos- phamide. The mice were administered intragastically with same dose of Hedysari Radix and Astragali Radix alternative Buzhong Yiqi Yiqi Yangxue, Yupingfeng oral liquid and Fuqi Zhihan granules for antagonistic experiments in vivo. And spleen index, HCs0, CD19 ＋ B lymphocyte subgroup and content of serum IL-4 were determined after treatment. Results ： Both groups of Hedyseri Radix and Astragali Radix could antagonize immunosuppressive action caused by cyclophosphamide. They both could significantly raise spleen index, HCs0, CD19＋ B lymphocyte subgroup and content of serum IL-4 in different degree. And Yupingfeng aqueous extract of Hedysari Radix substitute Astragali Radix was better than Yupingfeng oral liquid in raising spleen index. There were no significant differences among the rest Hedysari Radix and Astragali Radix alternative groups. Conclusion：Hedysari Radix compatibility with other drugs compared with original prescription has similar role in humoral immunity regulation.

关 键 词：红芪 黄芪 复方 免疫抑制 体液免疫 

分 类 号：R285.5[医药卫生—中药学]