毛茛总苷对肾性高血压大鼠血压、NO、AngⅡ及A7r5细胞内钙的影响  

Effect of TGRJ on Blood Press,NO and AngⅡ in Renal Hypertensive Rats

作　　者：刘守钾[1] 刘苑[1] 张兴[1] 杨智承[1] 刘威振[1] 谭毓治[1] 

机构地区：[1]广东药学院药科学院药理学教研室,广东广州510006

出　　处：《中药材》2012年第6期953-957,共5页Journal of Chinese Medicinal Materials

基　　金：广东省科技厅课题(2004B30101002)

摘　　要：目的:探讨毛茛总苷对肾性高血压大鼠的血压、血清一氧化氮(NO)、血浆和心肾组织中血管紧张素Ⅱ(AngⅡ)以及血管平滑肌细胞内钙的影响。方法:采用二肾一夹(2K1C)肾性高血压大鼠模型,连续灌胃给药5 w,5w末测定血压,检测血清中一氧化氮、血浆和心、肾组织中AngⅡ含量;采用新一代Ca2+荧光探针Fluo-3/AM标记培养的血管平滑肌细胞内钙,荧光倒置显微镜观察荧光成像和荧光分光光度计测量细胞内钙荧光强度,观察毛茛总苷对大鼠血管平滑肌细胞内Ca2+水平的影响。结果:毛茛总苷高、中剂量能降低肾性高血压大鼠血压水平(P<0.05),其各剂量均能显著的升高大鼠血清中NO含量(P<0.01),但对大鼠体内的AngⅡ含量无显著影响;毛茛总苷各剂量均能降低由AngⅡ引起的细胞内钙离子浓度升高。结论:毛茛总苷能降低肾性高血压大鼠的血压,其降压作用可能与其能显著的升高大鼠NO水平及拮抗AngⅡ引起的血管平滑肌细胞内钙升高有关。Objective：To study the effect of total glycosides of Ranunculus japoniucus （TGRJ） on blood pressure, nitric oxide（NO） calcium and angiotensin Ⅱ （ Ang Ⅱ ） in renal hypertensive rats （RHR）. Methods ： 1 ） RHR were established by two kidney one clip （2K1 C） and drugs were given by intragastric administration for 5 week, the blood pressure were measured at the end of 5 week, detected the concentration of NO in the serum and AngⅡ in the blood plasma, heart and kidney tissue. 2） Used a new generations of Ca2＋ fluorescent probe （Fluo-3/AM） to mark calcium in vascular smooth muscle cells. Observed the fluorescence imaging by inverted fluorescence microscope and measured the fluorescence intensity of calcium by fluorescence spectrophotometer in vascular smooth muscle cells; Results ： 1 ） The high, medium doses of TGRJ could decrease blood pressure of RHR （ P 〈 0.05 ）, TGRJ could significantly increase the concentration of NO in the serum（P 〈 0. 01 ）, but it showed no significant effect on the concentration of Ang Ⅱ （ P 〉 0. 05 ） ; 2） The AngⅡ could significantly promote the calcium ions in extracellular inner flow. The different doses of TGR.I could reduce the calcium ions in ceils which were mediated by Ang Ⅱ. Conclusion：TGRJ could decrease blood pressure in RHR. the mechanism might be related to increasing the rats＇ NO level and reducing the calcium ions level in cells which are increased by Ang Ⅱ.

关 键 词：毛茛总苷 肾性高血压 一氧化氮 血管紧张素 血管平滑肌细胞 

分 类 号：R285.5[医药卫生—中药学]