Altered Expression of Transcription Factor Genes in Rice Flag Leaf under Low Nitrogen Stress  

作　　者：ZHAO Ming-hui ZHANG Wen-zhong MA Dian-rong Xu Zheng-jin WANG Jia-yu ZHANG Li CHEN Wen-fu 

机构地区：[1]Rice Institute,Shenyang Agricultural University/Key Laboratory of Northern Rice Biology,Genetics and Breeding,Ministry of Agriculture,Shenyang 110866,China

出　　处：《Rice science》2012年第2期100-107,共8页水稻科学（英文版）

基　　金：supported by the Agricultural Science and Technology Achievement Transformation Fund of Science and Technology Ministry of China(Grant No. 2010GB2B000077);the Special Fund forAgro-scientific Research in the Public Interest of theministry of Agriculture of China (Grant No.201203026)

摘　　要：The response of transcription factor genes to low nitrogen stress was studied to provide molecular basis for improving the absorption and utilization efficiency of nitrogen fertilizer in rice. The agilent rice genome arrays were used to study the varied expression of transcription factor genes in two rice varieties （SN 196 and Toyonishhiki） with different chlorophyll contents under low nitrogen stress. The results showed that a total of 53 transcription factor genes （35 down-regulated and 18 up-regulated genes at the transcription level） in flag leaves of super-green rice SN196 and 27 transcription factor genes （21 down-regulated and 6 up-regulated genes at the transcription level） in flag leaves of Toyonishiki were affected by low nitrogen stress. Among those nitrogen-responsive genes, 48 transcription factor genes in SN196 and 22 in Toyonishiki were variety-specific. There were overlapped transcription factor genes responded to low nitrogen stress between SN196 and Toyonishiki, with 1 up-regulated and 4 down-regulated at the transcription level. Distributions of low nitrogen responsive genes on chromosomes were different in two rice varieties.The response of transcription factor genes to low nitrogen stress was studied to provide molecular basis for improving the absorption and utilization efficiency of nitrogen fertilizer in rice. The agilent rice genome arrays were used to study the varied expression of transcription factor genes in two rice varieties （SN 196 and Toyonishhiki） with different chlorophyll contents under low nitrogen stress. The results showed that a total of 53 transcription factor genes （35 down-regulated and 18 up-regulated genes at the transcription level） in flag leaves of super-green rice SN196 and 27 transcription factor genes （21 down-regulated and 6 up-regulated genes at the transcription level） in flag leaves of Toyonishiki were affected by low nitrogen stress. Among those nitrogen-responsive genes, 48 transcription factor genes in SN196 and 22 in Toyonishiki were variety-specific. There were overlapped transcription factor genes responded to low nitrogen stress between SN196 and Toyonishiki, with 1 up-regulated and 4 down-regulated at the transcription level. Distributions of low nitrogen responsive genes on chromosomes were different in two rice varieties.

关 键 词：RICE flag leaf MICROARRAY real-time quantitative PCR transcription factor low nitrogen stress 

分 类 号：S513[农业科学—作物学] Q943.2[生物学—植物学]