γδT细胞在3型鼠肝炎病毒诱导的小鼠慢性病毒性肝炎中作用的探讨  

Role of gamma delta T cells in the MHV-3 induced chronic viral hepatitis

作　　者：陆玉蕾[1,3] 王晓晶[1] 严伟明[1] 王洪武[1] 罗小平[2] 宁琴[1] 

机构地区：[1]华中科技大学附属同济医院感染性疾病研究所感染病科,湖北武汉430030 [2]同济医院儿科 [3]广西医科大学附属肿瘤医院

出　　处：《中西医结合肝病杂志》2012年第4期226-228,I0001,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：国家重点基础研究发展计划"973计划"资助项目(No.2007CB512900)

摘　　要：目的:探讨在3型鼠肝炎病毒(MHV-3)诱导的鼠慢性病毒性肝炎模型中随着感染时间的延长肝脏γδT细胞在肝脏T细胞中的比例变化以及细胞因子IFN(干扰素)-γ的表达。方法:C3H/Hej小鼠腹腔注射10pfu(空斑形成单位)MHV-3建立小鼠慢性病毒性肝炎模型,应用流式细胞技术荧光分析法检测MHV-3感染后0天、5天、10天、15天、20天肝脏中γδT细胞在T细胞中的比例变化及其表达IFN-γ的比例。结果:随着MHV-3感染时间的延长,模型中肝脏γδT细胞在肝脏T细胞中的比例较0天逐渐升高(P<0.05),在感染后10天达到最高值。肝脏γδT细胞大量表达IFN-γ,在MHV-3感染10天后达到最高值。结论:感染MHV-3后C3H/Hej小鼠体内γδT细胞的数量增加并表达IFN-γ,提示γδT细胞参与了MHV-3诱导的小鼠慢性病毒性肝炎的发生发展过程。Objective：To investigate the contribution of γδT cells in murine hepatitis virus type 3（MHV-3） induced chronic viral hepatitis in C3H/Hej mice.Methods：MHV-3 induced chronic viral hepatitis model was adopted in this research.On 0,5,10,15,20 days post MHV-3 infection,liver γδ＋T cells were marked by fluorescent antibodies and detected through flow cytometry for its proportion within CD3＋T cells and to identify their IFN-γ expression.Results：By the time-course of MHV-3 infection,the proportion of liver γδ＋T in CD3＋T cells were markedly increased and peaked at 10 days post MHV-3 infection.Moreover,the proportion of IFN-γ-expressing γδ＋T was elevated significantly after infection and peaked at day 10.Conclusion：Liver γδT may play an important role in the pathological process of MHV-3 induced chronic viral hepatitis.

关 键 词：ΓΔT细胞 慢性病毒性肝炎 3型鼠肝炎病毒 干扰素-Γ 

分 类 号：R512.6[医药卫生—内科学]