MAP30基因转化烟草的研究  

Study on transformation of MAP30 gene in tobacco

作　　者：栾杰[1] 陈秀玲[1] 侯莉华 李小梅[1] 王傲雪[1] 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030 [2]大庆市教师进修学院,黑龙江大庆163311

出　　处：《东北农业大学学报》2012年第7期109-113,共5页Journal of Northeast Agricultural University

基　　金：高等学校博士学科点专项科研基金(20092325110001);教育部科学技术研究重点项目(211043)

摘　　要：通过转基因技术,利用农杆菌介导法,将pBIMAP30植物表达载体转化烟草,Kan筛选抗性苗,选择植株健壮、根系发达的抗性植株,提取叶片DNA进行PCR鉴定,获得阳性转基因烟草。采用RT-PCR方法证实MAP30基因能够在转录水平上正常表达。该研究为探讨具有药用价值的植物资源奠定理论基础,并为获得抗病毒、抗肿瘤功能性药物蛋白的植物新品系研发提供一定的技术支持。The MAP30 gene was introduced into tobacco cultivar by Agrobacterium tumefaciens-mediated transformation,and the transformation system was optimized,and then the transgenic plants were detected.The result of PCR and RT-PCR showed the gene MAP30 was expressed in transgenic plants,which indicated that MAP30 gene was transformed into tobacco.The transgenic plant could be used to produce MAP30 for treatments of anti-virus and anti-tumor in the future.

关 键 词：MAP30基因 烟草 农杆菌介导法 

分 类 号：S572[农业科学—烟草工业] Q785[农业科学—作物学]