地毯草根际固氮菌的分离及鉴定  

Isolation and identification of associative nitrogen-fixing bacteria in the rhizosphere of Axonopus compressus

作　　者：樊俊华[1] 张晓波[1,2] 赵艳[1] 

机构地区：[1]海南大学农学院,海口570228 [2]热带作物种质资源保护与开发利用教育部重点实验室,海口570228

出　　处：《东北农业大学学报》2012年第8期105-109,共5页Journal of Northeast Agricultural University

基　　金：海南省自然科学基金项目(310019)

摘　　要：结合气相色谱仪,利用乙炔还原等方法对地毯草（Axonopus compressus）根际固氮菌进行分离和鉴定。结果表明,从地毯草根际土壤中分离获得6株固氮菌株,菌株的固氮酶活性相差较大（92.5 C2H4~295.2 C2H4nmol.mL-1.h-1）,菌株hnN2及hnN6具有较高的固氮酶活性（〉200 C2H4nmol.mL-1.h-1）;菌株经鉴定分属Bacillus（2株）、Azotobacte（r 3株）和Pseudomona（s 1株）3个属。This study isolates and identifies associative nitrogen-fixing bacteria from the rhizosphere of Axonopus compressus by acetylene reduction assay（ARA） combined with gas chromatography（GC）.Six strains were isolated,and showed that nitrogenase activity was large variation between strains（from 92.5 C2H4 to 295.2 C2H4nmol.mL-1.h-1）.Strains hnN2 and hnN6 showed higher nitrogenase activity（200 C2H4 nmol.mL-1.h-1）.All strains belonged to Bacillus（2 strains）,Azotobacter（3 strains） and Pseudomonas（1 strains）,respectively.

关 键 词：地毯草 固氮菌 分离与鉴定 根际 

分 类 号：S154.3[农业科学—土壤学]