猪带绦虫CDC37基因及其蛋白结构特征的生物信息学分析  

Bioinformatics Analysis on Cell Division Cycle 37 Gene of Taenia solnium and Structural Characteristics of Its Coding Protein

作　　者：刘玉江[1] 陈晓睿[2] 黄江[3] 戴佳琳[3] 廖兴江[4] 

机构地区：[1]贵阳医学院多媒体形态学实验室,贵州贵阳550004 [2]贵阳医学院寄生虫学教研室,贵州贵阳550004 [3]贵阳医学院法医学教研室,贵州贵阳550004 [4]贵阳医学院物理学教研室,贵州贵阳550004

出　　处：《贵阳医学院学报》2012年第4期344-347,351,共5页Journal of Guiyang Medical College

基　　金：国家自然科学基金资助项目(30760227)

摘　　要：目的:分析和预测猪带绦虫细胞分裂周期蛋白37(Ts CDC37)基因及其编码蛋白的结构和特性,用于指导其生物学功能的实验研究。方法:利用生物信息学网站所提供的有关基因和蛋白序列和结构功能分析工具,以及专业的生物信息学软件包,从猪带绦虫成虫全长cDNA质粒文库中识别CDC37基因,对猪带绦虫CDC37基因编码的蛋白质进行生物信息学分析。结果:该基因全长1 203 bp,编码400个氨基酸,为全长基因。Genebank中与日本血吸虫的cell division side 37 homolog蛋白的一致性最高,达60%。相对分子量理论预测值为46 802.5 ku,预测其有6个主要的B细胞抗原表位,无亚细胞定位序列。结论:应用生物信息方法从猪带绦虫成虫cDNA文库中筛选出了猪带绦虫CDC37基因全长序列并预测得到其结构与功能方面信息。Objective： To analyze and predict the structural characteristics of cell division cycle 37（CDC37）gene of Taenia solnium（Ts） and its encoding protein,and so as to guide the study on its biological function.Methods： Tools provided by bioinformatics webs for analysis on the structure,function and sequences of related gene and protein and bioinformatics software packages were employed to identify TsCDC37 gene from cDNA plasmid library,and bioinformatics analysis on the encoding protein of CDC37 gene was carried out.Results： This gene was 1 203 bp in length and was a full length gene coding 400 amino acids.The coding protein had highest coherence（60%） with CDC37 homolog protein of Schistosoma Japonicum in GeneBank.Relative molecular weight was 46 802.5 ku.The protein was predicted to have 6 B cell epitopes and have no subcellular location sequence.Conclusions： The full length sequence of Ts CDC37 gene is screened from cDNA library of adult Taenia solnium by bioinformatics method.The information about the structure and function of CDC37gene and protein is obtained.

关 键 词：猪带绦虫 基因 蛋白质结构 序列分析 

分 类 号：R383.3[医药卫生—医学寄生虫学]