绿盲蝽危害对枣树叶片生化指标的影响  

Physiological indices of leaves of jujube(Zizyphus jujuba) damaged by Apolygus lucorum

作　　者：高勇[1] 门兴元[2] 于毅[2] 周洪旭[1] 

机构地区：[1]青岛农业大学农学与植物保护学院,山东省植物病虫害综合防控重点实验室,青岛266109 [2]山东省农业科学院植物保护研究所,济南250100

出　　处：《生态学报》2012年第17期5330-5336,共7页Acta Ecologica Sinica

基　　金：公益性行业科研专项经费项目(201103012);旱区作物逆境生物学国家重点实验室开放基金(CSBAA2011-12);山东省自然科学基金(ZR2012CM018);山东省“泰山学者”建设工程专项经费资助

摘　　要：绿盲蝽已在棉区爆发成灾,并逐渐向枣树等北方果树转移危害,已对我国北方果树生产造成严重威胁。绿盲蝽刺吸危害后,能诱导植物产生一系列生化反应。通过生物化学方法研究绿盲蝽不同危害程度对枣树叶片生化指标的影响,探讨受害枣树叶片对绿盲蝽危害的应激反应的变化规律。结果表明,受害枣树叶片内可溶性糖含量随着危害程度的加重先升高,后降低;蛋白质含量随受害程度的加重而呈现降低趋势;游离氨基酸含量随受害程度的加重逐渐升高。受害后枣树叶片内3种防御性酶随受害程度的加重发生不同程度的变化,超氧化物歧化酶(Superoxide Dismutase,SOD)的活性随着危害程度的加重先升高后下降;过氧化物酶(Peroxidase,POD)的活性总体升高;不同受害程度枣树叶片内过氧化氢酶(Catalase,CAT)的活性之间以及它们与未受害枣树叶片内过氧化氢酶(CAT)的活性之间均未达到显著性差异水平。总之,随着绿盲蝽危害程度的加重,枣树叶片内可溶性糖、蛋白质、游离氨基酸含量以及防御性酶活性发生了不同程度的变化,枣树叶片内除过氧化氢酶(CAT)的活性变化外,可溶性糖、游离氨基酸和蛋白质的含量变化以及超氧化物歧化酶(SOD)和过氧化物酶(POD)的活性变化与绿盲蝽危害胁迫有明显的关系,说明枣树叶片对绿盲蝽的危害胁迫产生了应激反应,且叶片内除过氧化氢酶(CAT)外的其他生化指标与绿盲蝽的不同危害程度有一定的相关性。研究结果为理解枣树受绿盲蝽危害后的应激反应机制提供了材料,对绿盲蝽可持续治理具有重要指导意义。Apolygus lucorum Meyer-Dtir （ Hemiptera ： Miridae） is an important type of pest, damaging various agricultural and forest crops such as cotton, fruits, and weeds. A. lucorum feeds on young buds, leaves, and various other tissues of host plants with its piercing and sucking mouthparts, and it is an important secondary insect pest in agricultural production in China. With the extensive cultivation of Bt cotton since 1997 in China, the wide-spread use of this insect-resistant cultivar has drastically reduced the need for insecticides to control many key lepidopteran pests. However, this reduction in insecticide use has led to increased population densities of various mirids, among which A. lucorum is considered as the most important pest in cotton production in northern China. The large-scale cultivation of fruits in recent years has provided abundant food and appropriate hibernacle for A. lucorum. Consequently, it has gradually become a trees, especially jujube （Zizyphus jujuba）, causing serious damage in orchards in northern China. A. lucorum, the young leaves of jujube （Zizyphus jujuba） showed many needle-like reddish-brown significant pest for fruit After being damaged by dots, which formed into irregular holes as the leaves grew. Growth of the floral buds ceased, and black spots appeared on the young fruit. Many studies have shown that pest damage induces plants to produce stress responses. Therefore, certain physiological indices in plants will change during the response to pest damage. To examine the responses of jujube leaves to insect damage, we conducted biochemical degrees by A. lucorum. analyses to study changes The soluble sugar content and free amino acids content increased changed with increasing severity of leaf in various physiological indices of jujube leaves damaged to different first increased and then decreased, while the protein content decreased with increasing severity damage. Superoxide dis of leaf damage. The activities of protective enzymes also mutase （SOD） activity fi

关 键 词：绿盲蝽 危害 生理指标 应激反应 

分 类 号：S436.65[农业科学—农业昆虫与害虫防治]