凹凸棒石粘土固定辣根过氧化物酶处理含酚废水  

Horseradish peroxidase immobilization in palygorskite-based material for phenol wastewater treatment

作　　者：鲍腾[1] 彭书传[1] 陈冬[1] 刘海波[1] 舒林[1] 

机构地区：[1]合肥工业大学资源与环境工程学院,合肥230009

出　　处：《环境工程学报》2012年第9期3179-3185,共7页Chinese Journal of Environmental Engineering

基　　金：教育厅安徽高校省级自然科学研究重点项目(KJ2010A060);安徽省自然科学基金项目(11040606M99);安徽省科技成果转化基金项目(1104b0303023)

摘　　要：采用凹凸棒石粘土、可溶性淀粉和工业水玻璃作为制备凹凸棒石粘土基颗粒的材料,并将制备的凹凸棒石粘土基颗粒进行改性,得到了改性后的凹凸棒石基多孔材料,并将其作为固定辣根过氧化物酶的载体,再应用于含酚废水的处理研究,取得了良好的效果。实验结果表明,辣根过氧化物酶的最佳固定化条件为:单位酶活载体量1 mg、固定化时间1.5小时、固定pH值5。并且在固定化酶循环使用6次后,苯酚去除率仍能达到62.3%。Palygorskite clay, soluble starch and commercial silicate can be used to prepare a type of palygorskite-based granulated material. The granulated material was modified by modified palygorskite-based porous materials. The excellent performance of the porous materials applied in phenol wastewater removal was proved af ter horseradish peroxidase were fixed on the materials. The experimental results show that 1 mg unit volume of activity vector fixed time of 1.5 hours and pH value of 5 will ensure the best immobilization condition of horseradish peroxidase. After 6 times recycle test of the immobilized enzyme, phenol removal rate can reach 62.3%. Key words palygorskite clay; immobilization; horseradish peroxidase （HRP） ; phenol wastewater

关 键 词：凹凸棒石粘土 固定化 辣根过氧化物酶 含酚废水 

分 类 号：X783.4[环境科学与工程—环境工程]