胰岛素样细胞生长因子-1、碱性成纤维细胞生长因子及转化生长因子α对人表皮干细胞增殖的影响  

Effects of insulin-like growth factor-1,basic fibroblast growth factor and transforming growth factor α on the proliferation of human epidermal stem cells

作　　者：陈建国[1] 孟庆楠[1] 赵德梅[1] 谭谦[1] 

机构地区：[1]南京大学附属鼓楼医院整形烧伤外科,南京医学硕士研究生210008

出　　处：《医学研究生学报》2012年第8期789-792,共4页Journal of Medical Postgraduates

基　　金：江苏省"333高层次人才培养工程"科研项目(NJGL-2007116)

摘　　要：目的表皮干细胞是组织工程化皮肤的"种子细胞"。文中研究碱性成纤维细胞生长因子(basic fibroblast growthfactor,bFGF)、胰岛素样细胞生长因子-1(insulin-like growth factor-1,IGF-1)和转化生长因子α(transforming growth factorα,TGFα)对人表皮干细胞增殖的影响。方法采用两步酶消化法和Ⅳ型胶原差速贴壁相结合的方法获得人原代表皮干细胞,将表皮干细胞分为A组(K-SFM)、B组(K-SFM+bFGF)、C组(K-SFM+IGF-1)及D组(K-SFM+TGFα)进行培养。比较不同组之间表皮干细胞的克隆形成率、生长增殖、生长曲线和细胞周期等指标。结果 B组、C组及D组培养的表皮干细胞在细胞增殖、细胞克隆率方面检测结果均明显高于A组(P<0.05);流式细胞仪检测B组、C组与A组及D组处于G0/G1细胞比例比较差异有统计学意义(P<0.05)。结论 IGF-1、bFGF及TGF-α均对表皮干细胞的增殖有促进作用,IGF-1及bFGF对表皮干细胞表型的维持有较好的作用。Objective Epidermal stem cells are ＂seed cells＂ of the tissue-engineered skin. We studied the effects of IGF-1, bFGF and TGF a on the proliferation of epidermal stem cells. Methods We obtained primary epidermal stem cells using the two- step enzyme digestion and type-IV collagen coated chosen methods. We cultured the epidermal stem cells in K-SFM （group A）, K-SFM ＋ 10 ng/ml bFGF （group B）, K-SFM ＋ 10 ng/ml IGF-1 （group C） and K-SFM ＋ 10 ng/ml TGF-a （group D）, and compared their proliferation ability, cell growth curve, colony-forming rate and cell cycle in different media. Results The proliferation ability and colony-forming rate of the epidermal stem cells were significantly higher in groups B, C and D than in A （P 〈 0. 05 ）, and so was the proportion of the cells of the G0/G1 phase in groups B and C than in A and D （ P 〈 0. 05 ）. Conclusion IGF-1, bFGF and TGF a promote the proliferation of epidermal stem cells, and IGF-1 and bFGF can effectively maintain their phenotype.

关 键 词：胰岛素样细胞生长因子-1 碱性成纤维细胞生长因子 转化生长因子Α 表皮干细胞 增殖 

分 类 号：Q28[生物学—细胞生物学]