创伤性脑损伤后大鼠脑组织S100A6的表达规律及意义  

Expression and significance of S100 alpha 6 protein in rat brain tissues after traumatic brain injury

作　　者：房博[1,2] 贺晓生[2] 陈延[1] 梁明[2] 陈晓燕[2] 袁志海[1] 

机构地区：[1]延安大学附属医院神经外科,陕西延安716000 [2]第四军医大学西京医院神经外科,陕西西安710032

出　　处：《中华神经外科疾病研究杂志》2012年第4期336-338,共3页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目(30971173)

摘　　要：目的探讨大鼠创伤性脑损伤(traumatic brain injury,TBI)后脑组织中钙结合蛋白S100alpha 6(S100A6)的表达规律及意义。方法雄性SD大鼠54只,按随机数字表法分为正常对照组(6只)和TBI组(48只);采用头颅侧向旋转致伤方法制作TBI模型,并按伤后大鼠处死时间分为1,3,6,12,24,72 h、7 d,14 d共8个亚组,每组6只。采用免疫组化方法法检测S100A6蛋白在大鼠脑组织中表达规律及分布情况。S100A6阳性结果判断标准以细胞浆内出现棕黄染色颗粒为阳性,反之为阴性,为消除背景染色的影响,同一组织连续切片设阴性对照组。结果大鼠脑组织中S100A6蛋白在皮层与海马组织细胞的核膜、胞质、细胞膜中均有表达,脑损伤后一小时时间点皮层与海马细胞表达均最低(P<0.01),随后表达量逐渐递增,至损伤后7d其表达量恢复正常值,7 d、14 d与正常对照组比较,差异无统计学意义(P>0.05)。结论在TBI后,S100A6蛋白出现一个表达低峰,提示在不同机制影响下可能对脑损伤的发生与发展发挥重要作用。Objective To investigate the expression and significance of S100 alpha 6 protein（S100A6） in the brain of rat model of traumatic brain injury（TBI）.Methods Fifty-four male SD rats were divided randomly into normal control group（n=6） and TBI group（n=48）.The TBI model was created by using lateral head rotation device and subdivided into 1 h,3 h,6 h,12 h,24 h,72 h,7 d and 14 d group（6 rats in each group）.Theexpression and distribution of S100A6 in the rat brains were investigated by immunohistochemistry.The expression of the brown stained particles was considered as ＂positive＂ and lack of the stained particles as ＂negative＂.In order to estimate the contribution of the non-specific interaction binding,a negative antibody control was used in each serial sections.Results S100A6 was distributed on nucleus envelope,cytoplasm and cell membrane both in thehippocampus and the cortex.The expression of S100A6 was decreased to the lowest level in the rat brain at 1 h post TBI（P0.01）,then it became stronger gradually and recovered to normal level at 7 d.There was no statistical difference in the expression of S100A6 at 7 d and 14 d compared with the normal control group（P0.05）.Conclusion The lowest expression of S100A6 after TBIindicates that S100A6 may play an important role in the development of brain injury under the different mechanisms.

关 键 词：创伤性脑损伤 海马 皮层 S100A6 

分 类 号：R651.1[医药卫生—外科学]