GC法同时测定伊痛舒注射液中4种成分的含量  

Simultaneous determination of four compounds in Yitongshu injection by GC

作　　者：牛思佳[1] 陈霞[1] 赵明[1] 哈迪[2] 赵春杰[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]香港理工大学建设及环境学院,香港九龙999077

出　　处：《沈阳药科大学学报》2012年第9期685-688,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立伊痛舒注射液中α-蒎烯、莰烯、柠檬烯和甲基丁香酚4种成分含量测定的方法。方法采用挥发油提取器,利用GC法进行测定。采用DB-1石英毛细管柱(30 m×0.25 mm,0.25μm),氢火焰离子化检测器,程序升温,流速为1.0 mL.min-1,进样量为1μL,分流比为10∶1,内标物为丁香酚。结果α-蒎烯、莰烯、柠檬烯和甲基丁香酚质量浓度分别在22.77～728.6 mg·L-1、5.069～162.2 mg·L-1、4.844～155.0 mg·L-1和65.58～2 099 mg·L-1内线性关系良好,平均回收率分别为(94.0±1.2)%、(93.3±1.6)%、(93.1±2.2)%和(92.4±1.6)%。结论该方法准确,重复性好,可为伊痛舒注射液的质量控制提供依据。Objective To establish a method for the simultaneous determination of α-pinene, camphene, limo- nene and methyl eugenol in Yitongshu injection （traditional Chinese medicines）. Methods The sample was extracted by the oil extractor and determined with capillary column DB-1 （30m×0.25mm.0.25μm） by GC coupled with ：flame ionization detector. Programmed temperature was performed at the flow rate of 1.0 mL.min^-1 with injection volume of 1μL. Split injection was conducted with split ratio of 10： 1. The in- ternal standard was eugenol. Results α-Pinene, camphene, limonene and methyl eugenol were linear in the range of 22. 77-728.6 mg .L^ -1,5.069-162. 2 mg .L ^-1,4. 844- 155.0 mg .L^ -1 and 65.58-2 099 mg .L -1, respectively. The average recoveries were（94. 0% ± 1.2） %, （93.3 ± 1.6） %, （93.1 ±2. 2） % and（92. 4 ± 1.6 ） %. Conclusions This method is accurate and reproducible for the determination of α-pinene, camphene, limonene and methyl eugenol. It can be used for the quality control of Yitongshu injection.

关 键 词：伊痛舒注射液 气相色谱法 Α-蒎烯 莰烯 柠檬烯 甲基丁香酚 含量测定 

分 类 号：R917[医药卫生—药物分析学]