腹泻病人隐孢子虫及其他原虫感染粪样检测分析  

Investigation and fecal specimens detection of cryptozoite and other protozoon infection from patients with diarrhea

作　　者：陈韶红[1] 艾琳[1] 田利光[1] 张永年[1] 童小妹[1] 李浩[1] 陈家旭[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,卫生部寄生虫病原与媒介生物学重点实验室,世界卫生组织疟疾,血吸虫病和丝虫病合作中心,上海200025

出　　处：《中国人兽共患病学报》2012年第8期815-819,共5页Chinese Journal of Zoonoses

基　　金：国家重大传染病专项(2008ZX10004-001和2008ZX10004-011)联合资助~~

摘　　要：目的检测分析腹泻病人粪便中隐孢子虫及其他原虫感染情况,为该病的诊断、防治提供科学依据。方法用直接涂片法、碘液染色、改良抗酸染色法和金胺酚染色法作为病原学检测的金标准,同时联用ELISA、免疫试条法和FTA巢式PCR法,对临床109例腹泻病人隐孢子虫及其他原虫感染情况进行粪样检测分析。结果 109例腹泻病人中男性为65人,女性为44人,年龄在6～65岁,原虫检出率达到45%(49/109),感染对男女年龄无显著性差别(P>0.05)。其中,用直接涂片法和碘液染色法检测阿米巴原虫感染的阳性率为2.8%(3/109),人芽囊原虫感染的阳性率为34.9%(38/109),没有查到蓝氏贾第鞭毛虫;改良抗酸染色法加金胺酚染色法,隐孢子虫感染的阳性率为8.3%(9/109),微孢子虫阳性率为1.8%(2/109);隐孢子虫ELISA试剂盒法检测隐孢子虫感染的阳性率为6.4%(7/109);隐孢子虫免疫试条法检测的隐孢子虫感染的阳性率为1.8%(2/109);巢式PCR法检测隐孢子虫感染的阳性率达到9.1%(10/109),经过测序后为微小隐孢子虫和猫隐孢子虫。结论联合多种检测手段能提高肠道原虫的检出率,腹泻病人感染的原虫主要为隐孢子虫和人芽囊原虫。In order to understand the cryptozoite and other protozoon infection in the population of diarrhea patients,the methods of direct smear,iodine staining,auramine phenol staining and modified acid-fast stain were used as the gold standards for etiological diagnosis as well as the combination of ELISA,immunological test strips and FTA nest-PCR to detect cryptozoite and other protozoon infection situation from stool of 109 clinical cases of diarrhea patients.There were 65 male and 44 female with the age between 6 to 65 years old in the 109 investigated patients and the protozoon infection rate was as high as 45%（49/109）.Moreover,there was no significant difference between male and female（P0.05）.When it comes to the different detection methods,the positive infection rate were 2.8%（3/109） of amebic,34.9%（38/109） of Blastocystis hominis by direct smear and iodine staining method,respectively.Giardia lamblia was not detected.Meanwhile,the infection rate of cryptozoite was 8.3%（9/109） and microsporidian was 1.8%（2/109） by modified acid-fast stain with auramine phenol method.In the detection of cryptozoite,the positive rates were 6.4%（7/109） by ELISA,1.8%（2/109） by immunological test paper,and 9.1%（10/109） by FTA nest-PCR.After sequencing,the species have been affirmed as Cryptosporidium parvum and Cryptosporidium felis.In this investigation,cryptozoite and Blastocystis hominis were the main species.

关 键 词：腹泻 原虫感染 检测 隐孢子虫 

分 类 号：R382.2[医药卫生—医学寄生虫学]