致死型约氏疟原虫感染BALB/c小鼠巨噬细胞的作用地位研究  

A role of macrophages on immune responses in BALB/c mice infected with lethal Plasmodium yoelii 17XL

作　　者：冯辉[1] 潘艳艳[1] 李莹[1] 曹雅明[1] 

机构地区：[1]中国医科大学基础医学院免疫学教研室,沈阳028042

出　　处：《中国人兽共患病学报》2012年第8期828-831,共4页Chinese Journal of Zoonoses

基　　金：高校博士点专项基金(No.20112104110018)资助~~

摘　　要：目的固有免疫在疟疾急性期感染中的保护性作用地位有待深入研究。本研究旨在探讨巨噬细胞在致死型约氏疟原虫(Plasmodium yoelii 17XL,P.yoelii 17XL)感染中的作用地位。方法雌性BALB/c小鼠在感染-2、0d腹腔注射氯磷酸脂质体(clodronate liposomes,300μL/只/次),建立巨噬细胞消除模型。对照组小鼠在同时点注射同体积PBS脂质体。随后,小鼠腹腔注射1×106 P.y17XL寄生的红细胞。计数原虫血症和观察生存率。ELISA法检测脾培养上清IFN-γ和IL-10水平,Griess法检测NO(NO2-)水平。脾细胞流式细胞术(FACS)染色,计数F4/80+巨噬细胞数量。结果与对照组相比,巨噬细胞消除组原虫血症峰值提前出现,较对照组死亡时间提前。在感染0、3、5d,巨噬细胞消除组脾巨噬细胞占脾细胞百分含量和绝对值均明显降低。脾培养上清中IFN-γ、IL-10和NO分泌水平明显下降。巨噬细胞消除后加速P.y17XL感染BALB/c小鼠的感染进程且明显影响疟疾感染的最终结局。结论巨噬细胞在P.y17XL感染BALB/c小鼠中发挥重要的免疫保护作用。The role of the innate immune responses in the acute phase of malaria infection remains unclear.This study aims to investigate the role of macrophages in lethal Plasmodium yoelii 17XL（P.yoelii 17XL） infection.Female BALB/c mice were injected intraperitoneally with clodronate liposomes at-2 and 0 d of P.yoelii 17XL infection（300 μL/mouse/one time） to depleted macrophages in vivo.Control group of mice were injected intraperitoneally with the same volume of PBS liposome at the same time.Subsequently,the mice were intraperitoneally inoculated with 1 × 106 P.yoelii 17XL infected red blood cells.The parasitemia and survival rates were observed.The levels of spleen culture supernatant of IFN-γ and IL-10 were measured by ELISA.The level of NO（NO-2） was measured with the Griess assay.The numbers of F4/80＋ macrophages were detected by flow cytometry（FACS）.Compared with the control group,the peak parasitemia came out earlier and mice died earlier in macrophages depleted group.On the 0,3,and 5 d after infection,the percentage and absolute numbers of macrophages in spleen cells were significantly decreased in depleted group.Spleen culture supernatant of IFN-γ,IL-10 and NO secretion levels decreased significantly.It seemed that depletion of macrophages resulted in accelerating P.yoelii 17XL infection process and affected the outcome of the infection process of P.yoelii 17XL infection in BALB/c mice.It＇s indicated that macrophages play an important role in immune protection against P.yoelii 17XL infection in BALB/c mice.

关 键 词：致死型约氏疟原虫 巨噬细胞 固有免疫应答 

分 类 号：R328.3[医药卫生—人体解剖和组织胚胎学]