延迟5分钟剖宫产造全脑缺氧缺血新生大鼠模型  

A neonatal rat model of global brain hypoxic-ischemic injury established by 5-minute-delayed cesarean section delivery

作　　者：李星儿[1] 袁青[1] 唐纯志[1] 陈飞[1] 赵蓉[1] 吴加利[1] 黄燕彬[1] 孙铄[1] 刘祎思[1] 谢津津[1] 

机构地区：[1]广州中医药大学针灸推拿学院,广州510405

出　　处：《中国实验动物学报》2012年第4期21-25,I0001-I0003,共8页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(编号:81072878)

摘　　要：目的建立围产期全脑缺氧缺血性损伤的新生大鼠模型。方法 SD雌性大鼠妊娠21 d时,颈椎脱臼法处死,用止血钳夹闭双侧子宫角血管5 min后,剖宫产取出新生大鼠,交由代乳鼠喂养。结果造模组雌性大鼠9只,共娩出新生大鼠91只,出生3 d内死亡7只,死亡率7.7%。新生大鼠出生第2天进行翻身实验,第14天进行悬吊实验和斜坡实验,造模组和其余各组均有显著性差异。新生大鼠出生后21 d,取脑组织切片行HE染色,显示大脑皮层典型的缺氧缺血性损伤,与正常组相比,可见神经细胞明显的病理形态学改变。结论采用延迟5min剖宫产和代乳鼠喂养的方法,操作简便,并结合行为学测试筛选行为异常者,可建立稳定可靠、可供长期实验使用的围产期全脑缺氧缺血性损伤的新生大鼠模型。Objective To establish a neonatal rat model of global brain hypoxic-ischemic injury.Methods Timed pregnant Sprague-Dawley rat dams were decapitated on the expected day of delivery（gestation day 21）,hemostats were used to occlude the four vessels of cornu uteri for five minutes,then the pups were delivered by cesarean section and introduced to the surrogate dams for cross foster.Results 7 rat pups died within 3 days after born in 91 rat pups delivered by Delayed C-section（death rate was 7.7%）.The neuroethology of rat pups were evaluated by behavioral tests such as righting reflex test（P2d）,trapeze test and inclined plane test（P14d）.At the last,21-day-old rat pups were killed by decapitation and the brains collected for HE staining.Delayed C-section group was suggested to have significant difference with the control groups on both behavioral tests and HE staining pictures.Conclusion Delayed C-section delivery with cross fostered by surrogate dams can be an easy method to make a neonatal rat model of global brain hypoxic-ischemic damage for long-term experiments.

关 键 词：延迟剖宫产 缺氧缺血性脑损伤 妊娠大鼠 代乳鼠 新生大鼠 

分 类 号：Q95-33[生物学—动物学]