副溶血弧菌毒力评价小鼠模型的建立与应用  

Establishment and application of a mouse infection model for evaluating the virulence of Vibrio parahaemolyticus

作　　者：郭李平[1,2] 徐镔蕊[1] 杜德燕[2] 姜汉雯[2] 邱业峰[3] 周冬生[2] 

机构地区：[1]中国农业大学动物医学院,农业部动物流行病学与人兽共患病重点实验室,北京100193 [2]军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京100071 [3]军事医学科学院实验动物中心,北京100071

出　　处：《中国实验动物学报》2012年第4期31-34,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(30930001、30900823);973项目(2009CB522604)

摘　　要：目的建立用于评价副溶血弧菌毒力的小鼠模型,为研究副溶血弧菌的致病机制奠定基础。方法将适宜浓度的菌液经腹腔感染4～5周龄雌性BALB/c小鼠,观察小鼠的症状及死亡数。结果高盐(2%NaCl)条件下培养的强毒株RIMD2210633,经腹腔感染107CFU的菌量,小鼠存活率为20%～30%,而环境无毒株S251的小鼠存活率为100%。结论建立了评价副溶血弧菌毒力的实验小鼠模型,并应用于不同盐分浓度培养的强毒株与环境无毒株的毒力比较实验。Objective To establish a mouse infection model for evaluating the virulence of Vibrio parahaemolyticus.Methods 4-5-week old female Balb/c mice were monitored for symptoms and lethality after being infected intraperitoneally with Vibrio parahaemolyticus bacterial suspension.Results The survival rate of mice after i.p.infection with a highly virulent RIMD2210633 strain（107 CFU） cultivated in a high salt medium（2% NaCl） was 20-30%,while that with environmental non-virulent S251 strain was 100%.Conclusions We have successfully established a mouse infection model using the RIMD2210633 strain.This mouse model is suitable for evaluating the Vibrio parahaemolyticus virulence,and is used to compare the virulence of highly virulent and environmental non-virulent strains cultured in medium at different salt concentrations.

关 键 词：副溶血弧菌 小鼠 致死性 

分 类 号：Q95-33[生物学—动物学]