转人APP基因小鼠视网膜视神经细胞超微结构改变  

Ultrastructural changes in retinal neurons of APP transgenic mice

作　　者：卢艳[1] 董海莲[1] 王蓉[2] 

机构地区：[1]首都医科大学宣武医院眼科,北京100053 [2]首都医科大学宣武医院中心实验室,北京100053

出　　处：《中国实验动物学报》2012年第4期41-43,共3页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家"十一五"863计划"疫苗与抗体工程"子课题(2006AA02A245)(863生物标志物);北京市卫生系统高层次卫生技术人才培养计划资助(2009-3-64)资助

摘　　要：目的观察转人APP基因AD模型小鼠视网膜各层细胞超微结构的改变。方法实验采用C57BL/6J转人APP基因小鼠6只,采用同背景10个月龄的C57 BL/6J正常小鼠6只做为对照。灌注处死小鼠,完整取出右眼球分离视网膜,制作电镜标本观察视网膜各层细胞超微结构改变。结果与对照组相比,模型组视网膜各层细胞异常明显:膜盘排列结构模糊,局部间隙溶解、甚至消失;外核层细胞体干枯变形,染色质聚集浓缩;内核层可见固缩细胞;神经节细胞胞膜不完整,线粒体水肿,并可见染色质聚集。结论转人APP基因AD模型小鼠视网膜各层神经细胞超微结构存在明显病理改变。Objective To observe the ultrastructural changes in retinal neurons in APP transgenic mice.Methods We make comparison between six 10-month-old APP transgenic mice and 6 non-transgenic female mice of the same background and age.All mice were perfusion sacrificed.The whole right eyes were taken and processed for electron microscopic observation of changes in the retinal neurons.Results Compared with the control group,the APP transgenic mice group showed evidently pathological changes in neurons of each retina layers.The outer segment structure of cones and rods was blurring,there was cell pyknosis in the outer nuclear layer,cells were dried-up,chromatin was condensed in nuclear layer cells,ganglion cells showed disintegrated plasma membrane,cytoplasmic vacuoles and degenerative organelles,and mitochodria were severely swollen.Conclusions There are obviously pathological changes in the retinal neurons in APP transgenic mice.

关 键 词：APP转基因小鼠 阿尔兹海默病 电镜 视网膜神经细胞 

分 类 号：Q95-33[生物学—动物学]