高海拔地区内毒素致绵羊多脏器功能障碍综合征的病理学改变  

Pathological Characteristics of a Sheep Model of Multiple Organ Dysfunction Syndrome at High Altitude Induced by Lipopolysaccharide

作　　者：万东君[1] 牛廷献[2] 王春雨[3] 付学锋[1] 杨艳丽[4] 罗晓红[3] 

机构地区：[1]兰州军区兰州总医院干部病房,兰州730050 [2]兰州军区兰州总医院实验动物科,兰州730050 [3]兰州军区兰州总医院内分泌科,兰州730050 [4]兰州军区兰州总医院病理科,兰州730050

出　　处：《中国比较医学杂志》2012年第8期40-42,I0003,共4页Chinese Journal of Comparative Medicine

基　　金：甘肃省自然科学基金(No.1010RJZA052)

摘　　要：目的研究高海拔地区内毒素致绵羊多脏器功能障碍综合征(MODS)的病理学变化特征。方法将16只绵羊随机分为2组,中度海拔组和高海拔组,按相同的剂量静脉给予内毒素(6μg/kg)制作MODS模型,24h后麻醉处死绵羊,观察主要脏器的病理学变化。结果中度海拔和高海拔MODS组绵羊肺脏、肝脏、脾脏、肠粘膜均出现明显炎性改变,高海拔MODS组绵羊肺脏、肝脏病理学评分明显高于中度海拔MODS组。结论高海拔地区MODS的病理损伤较中度海拔明显加重。Objective To establish a sheep model of multiple organ dysfunction syndrome（MODS） at high altitudes induced by lipopolysaccharide（LPS） and observe the pathological characteristics of organs.Methods Sixteen 1-year old sheep（male∶female = 1∶1） were randomly divided into moderate altitude group（1500 m） and high altitude group（3200 m）.The MODS models were generated by intravenous infusion of LPS at a dose of 6 μg/kg.After 24 hours,the sheep were anesthetized,and samples of the lung,liver,heart,spleen,kidney and intestine tissues were taken and fixed in 10% formalin for histopathological examination.Results The lung,liver,spleen and intestinal tissues had significant inflammatory changes in both MODS groups,and especially,the pathology scores of the lung and liver at high altitude MODS group were significantly higher than those in the moderate altitude MODS group.Conclusions Pathological damages of MODS at high altitude are more aggravated than those at moderate altitude.

关 键 词：多脏器功能障碍综合征 内毒素 高海拔地区 绵羊 病理学 肺 肝 

分 类 号：R33[医药卫生—人体生理学]