高压氧对大鼠脊髓损伤后脊髓组织高迁移率族蛋白B1表达的影响  

Effect of hyperbaric oxygen on HMGB1 expression in the rat model of inflammatory responses after spinal cord injury

作　　者：杨晶[1] 王国忠[3] 刘雪华[1] 周毅[4] 王志为[2] 高春锦[1] 苏庆军[2] 

机构地区：[1]首都医科大学附属朝阳医院高压氧科,北京100020 [2]首都医科大学附属朝阳医院骨科,北京100020 [3]民航总医院高压氧科 [4]沈阳医学院沈洲医院骨科

出　　处：《中华航海医学与高气压医学杂志》2012年第4期193-197,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：北京市自然科学基金（7102062）

摘　　要：【摘要】目的探讨高压氧对大鼠脊髓损伤组织中高迁移率族蛋白B1（HMGB1）变化的影响及其对减轻脊髓损伤炎性反应的作用。方法160只成年健康SD大鼠，按数字表法随机平分为脊髓损伤组、脊髓损伤＋高压氧组、假手术对照组、假手术对照＋高压氧组4组，每组再随机平分为1、3、7、14d4个亚组，每亚组10只。在大鼠脊髓损伤后不同时间点，使用脊髓损伤大鼠功能恢复评分（BBB评分）评价后肢功能恢复情况，采用荧光定量PCR、Westernblot、免疫组化法，测定HMGB1、核因子KB（NF—KB）的表达。结果脊髓损伤后损伤组织HMGB1和NF—KB的mRNA及蛋白表达明显增加，差异具有统计学意义（P〈0．01）；高压氧干预后，脊髓损伤组织HMGB1mRNA及蛋白表达降低，1d和3d时差异无统学意义（P〉0．05），7d和14d时差异具有统计学意义（P〈0．05）；高压氧干预后，脊髓损伤组织NF．KBmRNA及蛋白表达降低，1d时差异无统计学意义（P〉0．05），3、7、14d时差异具有统计学意义（P〈0．05）；高压氧干预后，7、14dBBB评分明显改善，差异具有统计学意义（P〈0．05）。结论高压氧可降低HMGB1表达，减轻脊髓损伤继发炎性反应，促进大鼠神经功能的修复。Objective To investigate the effect of hyperbaric oxygen on high mobility group protein B1 （HMGB1） expression in the tissues of spinal cord injury, and the characteristic of secondary inflammatory response after spinal cord injury. Methods According to the randomization table, 160 healthy and adult Sprague-Dawley （SD） rats were equally divided into 4 groups, including the spinal cord injury group, the spinal cord injury ＋hyperbaric oxygen group, the sham operation group and the sham operation ＋ hyperbaric oxygen group. Each group was further randomly and equally divided into 4 subgroups： the 1~t, 3~d, 7th, 14th day groups. BBB score was used to evaluate functional rehabilitation of indlimb and the methods of PCR, Western blot, immunohistochemistry were performmed to determine the expressions of HMGB1 and nuclear factor kappa B （NF-KB） in the damaged tissues at different time points after spinal cord injury. Results The levels of mRNA and the expressions of protein of HMGB1 and NF-κB in the damaged tissues after spinal cord injury were significantly increased （P 〈 0.01 ）. By pre and posthyperbaric oxygen intervention, No significant differences the level of HMGB1 mRNA and the expression of HMGB1 protein after spinal cord injury （P 〉 0.05 ） on the 1 s, and 3rd days, but there were significant differences on the 7th and 14th days （ P 〈 0.05 ）. Hyperbaric oxygen intervention reduced the level of NF-κB mRNA and NF-κB protein expression, without significant difference （P〉0.05） on the 1st day, with significant differences （P 〈0.05） on the 3rd, 7th and 14th days. After hyperbaric oxygen intervention, the BBB scores were significantly higher on the 7th and 14th days （P 〈 0.05）. Conclusions Hyperbaric oxygen can reduce secondary inflammatory response after spinal cord injury by restricting the expression of HMGB1 and promoting the repair of neurological function.

关 键 词：高压氧 脊髓损伤 高迁移率族蛋白B1 

分 类 号：R651.2[医药卫生—外科学]