疏肝、健脾、疏肝健脾方对肝郁脾虚证模型大鼠消化系统的影响  

Influences of Formulae for Soothing Liver or Invigorating Spleen or Soothing Liver and Invigorating Spleen on Digest System of Syndrome Model of Stagnation of Liver and Deficiency of Spleen in Rats

作　　者：王玉杰[1] 谢鸣[2] 

机构地区：[1]厦门大学医学院中医系,福建厦门361005 [2]北京中医药大学国家重点方剂学学科,北京100029

出　　处：《中华中医药学刊》2012年第9期1957-1959,I0002,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(10002902-233-12)

摘　　要：目的:比较疏肝、健脾、疏肝健脾方对肝郁脾虚证模型大鼠消化系统的影响。方法:大鼠随机分为正常对照、肝郁脾虚证、柴胡疏肝散、四君子汤、柴疏四君汤5组,每组12只。后4组大鼠采用慢性束缚+过度疲劳+饮食失节法,连续4周造模,正常对照组不予处理,其中3个中药干预方组分别于造模第2周末,按10mL/kg给予相应方药灌胃,连续2周;模型组和正常对照组给予等量蒸馏水。于实验第29天,处杀各组大鼠,测定胃动素(MTL)、生长激素(SS)、胃蛋白酶、D-木糖排泄率、琥珀酸脱氢酶(SDH)、葡萄糖-6-磷酸酶(G-6-PD)。结果:模型组大鼠血浆MTL、SS及胃蛋白酶均见显著升高(P<0.05),D-木糖排泄率显著降低(P<0.05),肝脏SDH、G-6-PD均见显著升高(P<0.05)。柴胡疏肝散、四君子汤、柴疏四君汤3组大鼠的血清MTL均呈显著性降低(P<0.05);柴胡疏肝散、柴疏四君汤二组大鼠血清SS均见不同程度降低,四君子汤组显著升高(P<0.05);柴胡疏肝散、四君子汤、柴疏四君汤3组大鼠的D-木糖排泄率均呈不同程度升高,其中柴疏四君汤组和四君子汤组均呈显著升高(P<0.05);柴胡疏肝散、四君子汤、柴疏四君汤3组大鼠的胃蛋白酶均呈不同程度降低,其中柴疏四君汤组显著降低(P<0.05)。柴胡疏肝散、四君子汤、柴疏四君汤3组大鼠SDH均呈显著性降低(P<0.05);柴胡疏肝散、四君子汤、柴疏四君汤3组大鼠G-6-PD均见不同程度升高,其中柴疏四君汤组呈显著性升高(P<0.05)。结论:肝郁脾虚证模型大鼠存在消化功能紊乱,柴胡疏肝散、四君子汤、柴疏四君汤对肝郁脾虚证模型的消化功能均有不同程度的改善作用,其中以柴疏四君汤的作用最佳。疏肝健脾方与肝郁脾虚证之间具有更高的关联性。Objective ：To compare the influences of formulae for soothing liver or invigorating spleen or soothing liver and invigorating spleen on digest function in rat model of liver - stagnation and spleen - deficiency syndrome. Method ： All rats were randomly divided into the normal control group （ group 1 ） , model group of liver - stagnation and spleen - defi- eieney syndrome （ group 2 ） , Chaihu Shugan Powder group （ group 3 ） , Sijunzi Decoction group （ group 4 ） and Chaishu Sijun- zi Decoction group （group5） , 12 in eaeh group. The model was established in group 2,3,4 and 5 by chronic restraint -fa- tigue - diet disorder method for 4 weeks. Group 3,4 and 5 were given orally the relevant formulae（ 10mL/kg）for 2 weeks started at the end of the 2nd week,and group 1 and 2 were given equivalent distilled water. On the 29th day all rats were killed and the serum levels of MTL, SS, D - xyloseexeretion rate, pepsin, SDH and G - 6 - PD were detected. Result ： MTL and SS in plasma, pepsin, and SDH and G - 6 - PD in liver of model group increased significantly. D - xyloseexcretion rate in urine of model group decreased significantly. MTL in plasma of CHSGS, SJZT and CSSJT groups decreased signifi- cantly. SS in plasma of CHSGS aml CSSJT groups decreased in svarying degrees and that in SJZT group increased signifi- cantly. D - xyloseexe - retion rate in urine of CHSGS, $JZT and CSSJT groups increased in varying degrees and SJZT and CSSJT were significant. Pepsin of CHSGS,SJZT and CSSJT groups decreased in varying degrees and CSSJT was signifi- cant. SDH in liver of CHSGS, SJZT andCSSJT groups decreased significantly. G - 6 - PD in liver of CHSGS, SJZT and CSSJT groups increased significantly and CSSJT was significant.

关 键 词：方证相关 肝郁脾虚证 胃动素 生长激素 胃蛋白酶 D-木糖排泄率 琥珀酸脱氢酶 葡萄糖-6- 磷酸酶 柴胡疏肝散 四君子汤 柴疏四君子汤 大鼠 

分 类 号：R285.5[医药卫生—中药学]