滋补脾阴方药对脾阴虚糖尿病大鼠海马内质网应激影响的研究  

Effects of Zibu Piyin Recipe on Endoplasmic Reticulum Stress in Hippocampus of Rats with Diabetes of Spleen-Yin Deficiency

作　　者：梁丽娜[1] 常晓慧[1,2] 战丽彬[1,3] 施翔[4] 胡守玉 闫云[6] 郑路平[3] 张福良[7] 

机构地区：[1]大连医科大学附属二院中医科,辽宁大连116023 [2]中国人民解放军第210医院中医科,辽宁大连116021 [3]大连医科大学中西医结合研究院,辽宁大连116044 [4]潍坊市中医院老年病科,山东潍坊261041 [5]大连船舶康复医院神经内科,辽宁大连116013 [6]南阳市第一人民医院急诊科,河南南阳362018 [7]大连医科大学公共卫生学院,辽宁大连116044

出　　处：《中华中医药学刊》2012年第9期1969-1972,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30772847);高等学校博士学科点专项科研基金资助项目(20070161004);辽宁省高校优秀人才支持计划资助项目(LR2001015)

摘　　要：目的:探讨滋补脾阴方药(ZiBu PiYin Recipe,ZBPYR)对脾阴虚糖尿病大鼠海马内质网应激相关分子表达的影响。方法:高脂饲料喂养联合小剂量链脲佐菌素(streptozocin,STZ)注射建立2型糖尿病模型,在此基础上,通过复合因素造模的方法建立脾阴虚糖尿病模型,以水迷宫实验评价模型大鼠是否出现学习记忆障碍,之后观察各组大鼠海马内质网应激相关分子葡萄糖调节蛋白78(glucose regulated protein 78,GRP78)、蛋白激酶样内质网激酶(protein kinase RNA-like endoplasmic reticulum kinase,PERK)和真核转录因子2的α亚单位(eukaryotic translation initiation factor 2 subunitα,eIF2α)表达的变化。结果:ZBPYR治疗后GRP78 mRNA水平降低(P<0.05),GRP78、磷酸化PERK、磷酸化eif2α蛋白水平明显降低(P<0.05),与脾阴虚糖尿病组相比,差异有统计学意义。结论:滋补脾阴方药可能是通过影响内质网应激PERK信号传导改善脾阴虚糖尿病大鼠学习记忆障碍。Objective：To investigate the effects of Zibu Piyin Recipe（ZBPYR） on endop^asmic reticulum stress（ERS） - related protein expression in hippocampus of Spleen - Yin deficiency diabetic rats. Methods ： Type 2 diabetes model was established by high fat diet combined with low- dose streptozotocin（STZ）injection. Then we induced spleen -yin defi- ciency diabetic model by defatigation, hungry - full diet and drenching the drug of damaged yin. To evaluate whether dia- betic rats and spleen - yin deficiency diabetic rats were impaired in learning and memory, we performed Morris Water Maze test. We detected the expressions of GRP78, PERK and eIF2, which were related with ERS in cortex of rats. Re- sults ： After treating with ZBPYR, the protein levels of GRP78, phosphorylated PERK and phosphorylated eIF2 and the mR- NA expression of GRP78 were significantly decreased compared with Spleen - Yin deficiency diabetic rats. Conclusion： ZBPYR may improve learning and memory impairment in Spleen - Yin deficiency diabetic rats by affecting the endoplas- mie reticulum stress PERK signaling.

关 键 词：糖尿病认知下降 滋补脾阴方药 内质网应激 

分 类 号：R285.5[医药卫生—中药学]