红景Ⅰ号方对低氧大鼠阴茎海绵体平滑肌细胞表型转化的影响  

Protective Effects of Hongjing Yihao(HJYHF)on Phenotypic Modulation of Corpus Cavernosum Smooth Muscle Cells

作　　者：黄晓军[1] 吕伯东[1] 陈刚[1] 杨克冰[1] 张士更[1] 马寅锋[1] 张杰[1] 

机构地区：[1]浙江中医药大学附属第二医院泌尿男科,浙江杭州310005

出　　处：《中华中医药学刊》2012年第9期2027-2029,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江中医药管理局中医药重点项目(2009ZA009)

摘　　要：目的:观察红景Ⅰ方对低氧状态下大鼠阴茎海绵体平滑肌细胞表型转化的影响。方法:将120只SD大鼠随机分为常氧组、低氧模型组、前列腺E1组、红景Ⅰ号方低剂量组、红景Ⅰ号方中剂量组、红景Ⅰ号方高剂量组,每组20只,分别测定各组大鼠勃起情况、RT-PCR测定α-actin、OPN的相对表达量。结果:造模6周后,与常氧组比较,模型组勃起次数明显降低(P<0.01),低氧实验动物模型建立成功。与常氧组比较,低氧模型组的α-actin含量显著降低(P<0.01),OPN含量明显升高(P<0.01)。与低氧模型组比较,高中低剂量的红景Ⅰ号组α-actin含量上升(P<0.05),其中高中剂量组含量上升明显(P<0.01);OPN含量降低(P<0.05),其中高中剂量组含量下降明显(P<0.01)。结论:红景Ⅰ号方有抑制海绵体平滑肌细胞表型转化的作用。Objective：To observe effect of HJYHF Decoction on phenotypic modulation of corpus cavernosum smooth muscle cells （CCSMC）in rats under hypoxia. Methods ：120 SD rats were randomly divided into normal group, hypoxia mod- el group, prostate E1 group, the low - dose of HJYHF decoction group, the medium - dose of HJYHF decoction group,the high- dose of HJYHF decoction group. 20 in each group,the rats were measured erection. The expressions of α- actin and OPN were analyzed by RT -PCR. Results：After 6 weeks, compared with the normal group,the number of erection of model group significantly reduced（ P 〈 0.01 ） , hypoxic animal model was established successfully. Compared with normal group, the α - actin content of hypoxia model group was decreased （P 〈 0.01 ） , the content of OPN of hypoxia model group was increased（ P 〈 0.01 ）. Compared with the hypoxia model group, the α- actin content of the HJYHF Decoction group was risen（P 〈 0.01 ） ,the OPN content of the HJYHF Decoction group was reduced（ P 〈 0.01 ）. Conclusion：HJYHF De- coction can obviously prevent smooth muscle phenotypic modulation, which may be related with the impact of α - actin and OPN expressions.

关 键 词：红景Ⅰ号方 低氧 平滑肌表型转化 

分 类 号：R285.5[医药卫生—中药学]