复方楂金颗粒剂对非酒精性脂肪性肝炎大鼠氧化应激的影响  

Impact of Complex Prescription Zhajin Drug Granules(CPZD)on Oxidative Stress in NASH Rats

作　　者：蒋艳明[1] 陆璐[2] 钱建成[2] 昂健[3] 田静[4] 荀运浩[4] 施军平[1] 

机构地区：[1]杭州师范大学附属医院,浙江杭州310015 [2]浙江中医药大学,浙江杭州310053 [3]浙江大学医学院附属第二医院,浙江杭州310009 [4]浙江中医药大学附属杭州第六医院,浙江杭州310014

出　　处：《中华中医药学刊》2012年第9期2066-2068,I0004,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药计划科技项目(2008JA009);杭州市科技计划项目(2006533Q13)

摘　　要：目的:研究氧化应激对高脂高糖饮食诱导的NASH大鼠肝脏损伤的作用,观察复方楂金颗粒剂对NASH的治疗作用,探讨其作用机制。方法:以高脂高糖饮食喂养10周建立NASH大鼠模型,分别以4.032mg.kg-1.d-1、2.016mg.kg-1.d-1、1.008mg.kg-1.d-1的CPZD和69.2mg.kg-1.d-1的易善复进行治疗10周后,观察肝组织病理变化,血清和肝组织MDA含量以及SOD、GSH-PX活性。结果:与正常组相比,模型组大鼠肝脂肪变性、NAFLD活动度积分(NAS)均明显升高(P<0.05),肝组织SOD、GSH-PX活性明显降低而MDA含量明显增加(P<0.05),但血清SOD、GSH-PX活性差异无统计学意义(P>0.05)。改变饮食并应用CPZD治疗后,明显降低NASH大鼠NAS和肝组织MDA含量(P<0.05),升高肝组织SOD、GSH-PX活性(P<0.05),而肝细胞脂肪变性差异无统计学意义(P>0.05)。结论:氧化应激和脂质过氧化是高脂高糖饮食诱导的大鼠发生NASH主要机制,在改变饮食基础上,CPZD不能有效改善肝脂肪变性程度,但明显减轻NASH大鼠的氧化应激,抑制脂质过氧化反应。Objective ：To study oxidative stress influence on liver injury of the NASH rat induced by high fat and glu- cose diet,and observe the therapeutics effect of CPZD and explore its mechnism. Methods： The model rats of NASH were induced by 12 weeks＇high fat and glucose diet, and treated with CPZD and Essentiale respectively. The pathological chan- ges of rats liver were observed, the amounts of liver MDA, and the activities of SOD and GSH - PX were detected. Results ： Comparing with the normal control group, the degree of hepatocyte steatosis, NAFLD activity scores（NAS） and the content of MDA of liver significantly increased （ P 〈 0.05 ）, the activity of SOD and GSH - PX of liver significantly decreased （ P 〈 0.05 ）, but the activities of SOD and GSH - PX of serum were not significantly decreased. After changing diet and treated by CPZD, comparing with the model group, the NAS and the content of MDA of liver remarkly decreased（ P 〈 0.05 ） , the activities of SOD and GSH - PX of liver were significantly higher（ P 〈 0.05 ） ,but the degree of hepatocyte steatosis was not decreased （P 〉 O. 05 ）. Conclusion：Oxidation stress and lipid peroxidation exsisted in rats with NASH induced by high fat and glucose diet, and played an important role in the information and procedure of NASI-I. At the base of changing di- et, CPZD could significantly decrease oxidation stresssuppress,lipid peroxidation of the NASH rats and enhance the ability of anti - oxidation, which is the important mechanism of treatment for NASH.

关 键 词：非酒精性脂肪型肝炎 氧化应激 脂质过氧化 复方楂金颗粒剂 

分 类 号：R285.5[医药卫生—中药学]