Roux-en-Y胃转流术对2型糖尿病大鼠血清vaspin表达的影响  

Role of Vaspin in Roux-en-Y Gastric Bypass on Type 2 Diabetes Mellitus Rats

作　　者：唐锦[1] 魏寿江[1] 王崇树[1] 李勋[1] 何一[1] 肖江卫[1] 

机构地区：[1]川北医学院附属医院普外一科,四川南充637000

出　　处：《中国普外基础与临床杂志》2012年第8期823-827,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：四川省教育厅课题(项目编号:10ZC044)~~

摘　　要：目的研究Roux-en-Y胃转流术(RYGB)对2型糖尿病(T2DM)大鼠的治疗作用,并探讨内脏脂肪组织产生的丝氨酸蛋白酶抑制剂(vaspin)在RYGB治疗T2DM机制中的可能作用。方法取造模成功的T2DM大鼠20只和周龄、性别相匹配的正常SD大鼠20只,用随机数字表法将其随机分为T2DM-RYGB组、T2DM-假手术组及RYGB组、假手术组,每组10只。分别于术前及术后第4和8周检测各组大鼠的空腹血糖(FPG)、血清胰岛素(INS)、血清vaspin水平及胰岛素抵抗指数(HOMA-IR),并分析血清vaspin水平与FPG、INS及HOMA-IR的相关性。结果手术前,T2DM-RYGB组与T2DM-假手术组比较以及RYGB组与假手术组比较,FPG水平、INS水平、vaspin水平及HOMA-IR差异均无统计学意义(P>0.05);而T2DM-RYGB组及T2DM-假手术组的FPG水平、INS水平、vaspin水平及HOMA-IR均分别明显高于RYGB组(P<0.05)及假手术组(P<0.05)。术后第4周,T2DM-RYGB组FPG水平、INS水平、vaspin水平及HOMA-IR较术前下降,除FPG水平(P<0.05)外,其余指标与术前比较差异均无统计学意义(P>0.05);术后第8周,FPG水平、INS水平、vaspin水平及HOMA-IR进一步下降,与术前比较差异均有统计学意义(P<0.05)。T2DM-假手术组、RYGB组及假手术组组内术前及术后第4周、第8周FPG水平、INS水平、vaspin水平及HOMA-IR比较,差异均无统计学意义(P>0.05)。手术前及手术后第4周、第8周T2DM-RYGB组与T2DM-假手术组血清vaspin水平与其对应血清INS水平、HOMA-IR均呈正相关(P<0.05)。结论 RYGB对T2DM大鼠具有一定的治疗作用,RYGB后vaspin表达水平降低,胰岛素敏感性改善,这可能是RYGB治疗T2DM的机制之一。Objective To study the therapeutic effect of Roux-en-Y gastric bypass （RYGB） on type 2 diabetes mellitus （T2DM） rats and explore the possible mechanism of vaspin in RYGB on T2DM. Methods Twenty SD rats with T2DM and 20 age- and sex-matched normal SD rats were randomly divided into 4 groups according to the random digits table： T2DM-RYGB group, T2DM-sham operation （SO） group, RYGB group, and SO group, 10 rats in each group. Fasting plasma glucose （FPG） level, serum insulin （INS） level, vaspin level, and homeostasis model of insulin resistance （HOMA-IR） were determined before operation and on week 4, 8 after operation, respectively. At the same time, the correlation between vaspin and the indicators （FPG, INS, or HOMA-IR） was analyzed. Results Compared the indicators after operation with before operation, the FPG level, INS level, vaspin level, and HOMA-IR were not significantly different between the T2DM-RYGB group and T2DM-SO group （P〉0. 05） or between the RYGB group and SO group （P〉0. 05）, but the FPG level, INS level, vaspin level, and HOMA-IR in the T2DM-RYGB group and T2DM-SO group were significantly higher than those in the RYGB group （P〈 0. 05） and SO group （P〈 0. 05）, respectively. On week 4 after operation, the FPG level, INS level, vaspin level, and HOMA-IR decreased in the T2DM-RYGB group, except for the FPG level, the other indexes had no significant differences as compared with the values before operation. On week 8 after operation, the FPG level, INS level, vaspin level, and HOMA-IR further decreased in the T2DM-RYGB group, there were significant differences of these indicators between before operation and on week 8 after operation. Compared the indicators after operation with before operation, the FPG level, INS level, vaspin level, and HOMA-IR were not statistically significant （P〉0. 05） in the T2DM-SO group, RYGB group, or SO group. The changes in serum vaspin level correlated positively with those in INS and HOMA-IR before operaion and o

关 键 词：ROUX-EN-Y胃转流术 2型糖尿病 血糖 胰岛素 VASPIN 

分 类 号：R656.6[医药卫生—外科学] R587.1[医药卫生—临床医学]