猪FSHβ基因第2外显子多态性与产仔性能的关联分析  

Association between Polymorphism of Exon 2 of FSHβ Gene and Litter Sizes in Swine

作　　者：吴兆海[1] 薛振锋[1] 梁超[1] 刘宏[2] 薛尚军[2] 曹果清[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801 [2]大同市种猪场,山西大同037000

出　　处：《中国畜牧杂志》2012年第17期6-9,共4页Chinese Journal of Animal Science

基　　金：山西省攻关项目(991016和20080311031);山西省归国留学人员资助项目(2007066);山西农业大学大学生科技创新项目

摘　　要：采用PCR-SSCP技术检测马身猪、杜洛克猪、大白猪、山西黑猪和山西白猪等5个猪种421个个体FSHβ基因编码区的多态性,采用单变量动物模型分析了多态位点对母猪产仔性能的影响。结果表明:在FSHβ基因第2外显子第48位上检测到C→T的转换突变为同义突变。在杜洛克群体中,C等位基因的频率为1;在马身猪群体中,T等位基因的频率为1;在山西白猪和山西黑猪中,等位基因C的频率相对较高,分别为0.65和0.89。不同基因型母猪总产仔数和产活仔数的差异不显著(P>0.05),可初步判断该突变位点对猪的产仔性能无显著影响。A total of 421 pigs including Mashen, Duroc, Large White, Shanxi Black and Shanxi White pig populations were used to detect the polymorphism within the coding region of FSHfl gene by PCR-SSCP. The C48T transition mutation, a synonymous mutation,was found within exon 2 of FSHβ gene. The frequency of C allele was 1 in Duroc population, whereas the frequency of T allele was 1 in Mashen population. In Shanxi Black and Shanxi White populations, the frequencies of allele C were higher than those of T, which were 0.65 and 0.89, respectively. The character of the allele distribution at this polymorphic site was in accordance with pig types in reproduction performance. The univariate animal model was used to analyze the effects of the polymorphic site of FSHβ on total number born （TNB）and number born alive （NBA）at first parity. The results showed that there were no difference among sows with different FSHβ genotypes in TNB and NBA, which implied that the C48T mutation within exon 2 of FSHfl gene has no significant effect on litter size in swine.

关 键 词：FSHΒ基因 多态性 产仔数 猪 

分 类 号：S828.2[农业科学—畜牧学]