罗格列酮联合头孢他啶对脓毒症大鼠过氧化物酶体增殖物激活受体γ活性和白细胞介素的影响  

The effects of rosiglitazone combined ceftazidime on peroxisome proliferator activated receptor γ activity and interleukin in septic rats

作　　者：曾勉[1] 黄晓梅[2] 何婉媚[1] 陈虎[1] 蒋玉洁[1] 

机构地区：[1]中山大学附属第一医院MICU,广东广州510080 [2]珠海市人民医院呼吸内科

出　　处：《中国危重病急救医学》2012年第9期550-553,共4页Chinese Critical Care Medicine

基　　金：广东省科技计划项目

摘　　要：目的观察罗格列酮（RSG）和头孢他啶（CAZ）对脓毒症大鼠过氧化物酶体增殖物激活受体1（PPARγ）活性及白细胞介素（IL-4、IL-6）的影响。方法将180只sD大鼠按随机数字表法均分为正常对照组、假手术组、脓毒症组、CAZ组、RSG组、CAZ＋RSG组。采用盲肠结扎穿孔术（CLP）制备大鼠脓毒症模型，术后3h腹腔注射相应药物，12h1次，于术后12、24、48h采用酶联免疫吸附试验（ELISA）测定大鼠血中有核细胞PPARγ活性及血浆IL-4、IL-6水平。结果正常对照组与假手术组术后各时间点有核细胞PPARγ活性及IL-4、IL-6水平均无差异。脓毒症组有核细胞PPAR叮活性[吸光度（A）值]较正常对照组和假手术组明显降低（0．263±0．017比0．292±0．005、0．294±0．007，均P〈0．05），且随时间延长呈逐渐下降趋势；CAZ组、RSG组、CAZ＋RSG组PPARγ活性明显高于脓毒症组（0．282±0．008、0．336±0．020、0．347±0．007比0．263±0．017，均P〈0．05），其中CAZ＋RSG组〉RSG组〉CAZ组（均P〈0．05）。脓毒症组血IL-6（ng／L）、IL-4（ng／L）水平明显高于正常对照组和假手术组（IL-6：436．77±62．28比45．11±10．42、42．28±7．54，IL-4：89．24±25．06比41．34±7．08、41．49±7．27，均P〈0．05），分别于术后24h和48h达高峰；CAZ组、RSG组、CAZ＋RSG组IL-6、IL-4水平明显低于脓毒症组（IL-6：273．48±12．13、317．64±14．10、253．94±13．57比436．77±62．28；IL-4：59．12±7．03、68．37±8．28、53．81±8．34比89．24±25．06，均P〈0．05），其中CAZ＋RSG组〈CAZ组〈RSG组（均P〈0．05）。结论脓毒症大鼠有核细胞PPAR7活性下降，在应用抗菌药物（CAZ）进行有效抗感染的基础上使用RSG，可提高PPARγ活性，降低血浆炎症和抗炎介质水平。Objective To observe the effects of rosiglitazone （RSG） and ceflazidime （CAZ） on peroxisome proliferator activated receptor γ （PPARγ） activity in nucleated cells and interleukin （IL-4, IL-6） levels in plasma in septic rats. Methods According to randomized digital table, 180 male Sprague-Dawley （SD） rats were assigned to control group, sham operation group, sepsis group, CAZ group, RSG group and combined CAZ and RSG group. Sepsis model was established by cecal ligation and puncture （CLP）. Drugs were administered by intraperitoneal injection at 3-hour post-operation, once every 12-hour. The PPARγ activity in nucleated cells and IL-4, IL-6 levels in plasma were detected by enzyme linked immunosorbent assay （ELISA） at 12, 24 and 48 hours post-operation. Results There was no difference in PPARγ activity and levels of IL-4 and IL-6 at each time point post-operation between control group and sham operation group. Compared with control group and sham operation group, PPARγ activity [ absorbance （A） value] in nucleated cells in sepsis group, where downward trend was seen as time went on, significantly reduced （0.263 ± 0.017 vs. 0.292 ± 0.005, 0.294 ± 0.007, both P〈0.05）. PPARγ activity was significantly higher in CAZ group, RSG group and CAZ ＋ RSG group than in sepsis group （0.282 ± 0.008, 0.336 ± 0.020, 0.347 ± 0.007 vs. 0.263 ± 0.017, all P〈0.05）, CAZ ＋ RSG group〉RSG group 〉CAZ group （both P〈0.05）. Plasma IL-6 （ng/L） and IL-4 （ng/L） levels were higher in sepsis group than in control group and sham operation group （IL-6：436.77 ± 62.28 vs. 45.11 ± 10.42, 42.28 ± 7.54; IL-4：89.24 ± 25.06 vs. 41.34 ± 7.08, 41.49 ± 7.27, all P〈0.05） and reached peak at 24 hours and 48 hours post-operation, respectively. Compared with sepsis group, IL-6 and IL-4 levels in CAZ group, RSG group and CAZ ＋ RSG group were significantly decreased （IL-6：273.48 ± 12.13, 317.64 ± 14.10, 253.94 ± 13.57 vs. 436.77 ± 62.28; IL-4：59.12 ± 7.03, 68.37

关 键 词：罗格列酮 头孢他啶 脓毒症 过氧化物酶体增殖物激活受体Γ 配体 白细胞介素 

分 类 号：R459.7[医药卫生—急诊医学]