纳洛酮对弥漫性脑损伤合并二次脑损伤大鼠c-fos表达及β内啡肽水平的影响  

Effect of naloxone hydrochloride on c-fos protein expression in brain and plasma beta-endorphin level in rats with diffuse brain injury and secondary brain insult

作　　者：荆俊杰[1] 王守森[1] 杨庆武[2] 王如密[1] 高进喜[1] 赵琳[1] 

机构地区：[1]南京军区福州总医院神经外科,福州350025 [2]福州市第二医院神经外科,福州350001

出　　处：《解放军医学杂志》2012年第9期864-867,共4页Medical Journal of Chinese People's Liberation Army

摘　　要：目的观察应用纳洛酮干预后,弥漫性脑损伤(DBI)合并二次脑损伤(SBI)大鼠脑内c-fos蛋白及血浆β内啡肽(β-EP)的表达变化,以进一步探讨β-EP与c-fos在SBI中的作用。方法健康雄性SD大鼠70只,随机分为DBI合并SBI组(A组)、DBI合并SBI后盐酸纳洛酮(1mg/kg,腹腔注射)治疗组(B组)、DBI后SBI前盐酸纳洛酮治疗组(C组),于伤后3、24、48h处死大鼠,采用免疫组化及放射免疫分析法测定脑内c-fos蛋白和血浆β-EP的含量,并进行脑组织病理形态学观察。结果 B、C两组在各时间点的各检测指标比较差异均无统计学意义(P>0.05)。与B、C两组比较,A组3h及24h时的脑组织含水量明显升高(P<0.05),24h及48h时的神经元损伤数量明显减少(P<0.05),3h、24h及48h时的c-fos蛋白表达明显升高,3h及24h时的血浆β-EP含量也明显升高(P<0.05)。结论盐酸纳洛酮可降低DBI合并SBI大鼠脑内c-fos蛋白及血浆β-EP的含量,减轻神经损伤程度,具有一定的神经保护作用;DBI合并SBI后与DBI后SBI前使用盐酸纳洛酮对SBI的疗效相仿。Objective To observe the changes of c-fos protein expression in brain and beta-endorphin（β-EP） level in blood plasma in rats with diffuse brain injury（DBI） and secondary brain insult（SBI） after intraperitoneal injection of naloxone hydrochloride,and explore the role of c-fos andβ-EP in development of SBI in rats.Methods Seventy health male SD rats were enrolled in the present study and randomly divided into group A（intraperitoneally injected with 0.9% saline after DBI and SBI model was reproduced）,group B（injected intraperitoneally with 1.0mg/kg naloxone hydrochloride after DBI and SBI model was reproduced）,and group C（intraperitoneally injected with 1.0mg/kg naloxone hydrochloride after DBI and before SBI model was reproduced）.The animals were sacrificed 3,24 and 48 hours after injury,and the number of c-fos positive cells in brain and content of β-EP in blood plasma were determined by immunohistochemistry and radioimmunoassay respectively,the water content and number of injured neurons in brain tissue were measured by pathomorphological observation of the brain tissue.Results No significant difference was observed between group B and C for all the detection parameters.In group B and C,the water content in brain tissue at 3h and 24h was found to be decreased,while the number of injured neurons at 24h and 48h increased,number of c-fos positive cells in brain at 3h,24h and 48h decreased,and content of β-EP in blood plasma at 3h and 24h decreased when compared with group A（P〈0.05）.Conclusion Naloxone hydrochloride could decrease the c-fos expression in brain and β-EP level in blood plasma,alleviate the nerve injury,and protect neural function.The therapeutic effect of naloxone administered either after DBI and SBI or after DBI and before SBI was similar.

关 键 词：脑损伤 原癌基因蛋白质C-FOS Β内啡肽 纳洛酮 

分 类 号：R651.15[医药卫生—外科学]