丹参ACC氧化酶基因(SmACO1)的克隆与序列分析  

Cloning and Sequence Analysis of 1-Aminocyclopropane-1-Carboxylic Acid Oxidase Gene(SmACO1) from Salvia miltiorrhiza Bunge

作　　者：周露[1] 化文平[1,2] 王喆之[1] 李翠芹[1] 

机构地区：[1]教育部药用资源与天然药物化学重点实验室,西北濒危药材资源开发国家工程实验室,陕西师范大学生命科学学院,西安710062 [2]陕西教育学院生命科学与技术系,西安710100

出　　处：《植物研究》2012年第5期584-590,共7页Bulletin of Botanical Research

基　　金：陕西师范大学中央高校基本科研业务费重点项目(GK200901014)

摘　　要：依据丹参转录组数据库序列信息,采用RT-PCR和染色体步移技术从丹参中首次克隆得到ACC氧化酶基因,命名为SmACO1(GenBank注册号为JQ026111)。该基因gDNA序列长1 347 bp,由3个外显子和2个内含子组成;cDNA全长1 117 bp,包含945 bp的开放阅读框,编码314个氨基酸残基。生物信息学分析显示SmACO1为无信号肽与跨膜结构域,且定位于细胞质的稳定亲水蛋白,含有Fe2+依赖的加氧酶结构域。实时荧光定量PCR结果表明,SmACO1基因在丹参不同组织器官中差异表达,花中表达量最高;其表达受到病原菌和茉莉酸甲酯的诱导,表明SmACO1基因可能在植物防御反应中发挥作用。According to the transcriptome database of Salvia miltiorrhiza Bunge, a novel ACO gene was cloned with the method of RT-PCR and genome walking for the first time, and named as SmACO1 （ GenBank accession number： JQ026111）. The genomic sequence of SmACO1 is 1 347 bp in length, consisting of 3 exons and 2 introns. The full length of SmACO1 cDNA is 1 117 bp, and has an opening reading frame of 945 bp, which encodes 314 amino acids. Bioinformatics analysis showed that SmACO1 was a stable hydrophilic protein located in the cytoplasm without signal peptide and transmembrane domain, and contains a Fe （ II ）-dependent oxygenase superfamily domain. Quantitative RT-PCR analysis revealed that SmACO1 expressed differently in different or- gans and the expression level was the highest in flower. Furthermore, this gene could be induced by pathogen and methyl jasmonate, indicating that it might be involved in plant defenses.

关 键 词：丹参 ACC氧化酶 基因克隆 生物信息学分析 表达模式 

分 类 号：Q943.2[生物学—植物学]