山东省长岛县南部四岛藜(Chenopodium album L.)天然种群遗传多样性的ISSR分析  

Analysis on Genetic Diversity of Chenopodium album L. Populations in Four Natural Islands of South of Changdao,Shandong Province by ISSR

作　　者：张恒庆[1] 房孝迪[1] 吴锦美[1] 杜洋[1] 谭琨[1] 范强军 祝恩伟 

机构地区：[1]辽宁师范大学生命科学学院,大连116029 [2]山东长岛国家级自然保护区管理局,长岛265800

出　　处：《植物研究》2012年第5期632-635,共4页Bulletin of Botanical Research

摘　　要：采用ISSR分子标记对分布于山东省长岛县南部的北长山、南长山、大黑山和小黑山4个岛屿上的藜天然种群共81个个体进行了遗传多样性及遗传结构的研究。13个引物共检测到157个可重复的位点。遗传多样性研究结果表明:种内的多态位点比率为66.24%,具有较高的遗传多样性(Shannon(I)指数在物种水平上为0.332 0);种群间有一定的遗传分化,根据Gst值,种群间的遗传多样性占总群体的9.27%。遗传距离分析表明,XHS种群和NCS种群的遗传一致度最高,与地理距离无相关性。Using ISSR method, the genetic diversity and genetic structure of 81 individuals in four natural populations of Chenopodium album L. distributed in four islands in Changdao, Shandong Province, i.e. Beichangshan island, Nanchangshang island, Daheishan island and Xiaoheishan island were studied in this paper. Through the amplification of ISSR with 13 primers, 157 loci were detected. The results showed that the percentage of polymorphic loci was 66.24% with a high genetic diversity （ Shannon＇ s information index was 0. 332 0 at the species level） ; genetic differentiation existed among populations （ Nei＇ s gene diversity was 0. 220 1 at the spe- cies level） ; at the species level, genetic variance was 9.27% among populations according to GST value. The genetic distance analysis showed that XHS population and NCS population have the highest population genetic identity, genetic distances and geographic distances have no correlation.

关 键 词：藜 遗传多样性 遗传结构 ISSR 

分 类 号：Q949.745.1[生物学—植物学]