慢性浅表性胃炎脾虚与脾胃湿热证患者物质能量代谢基因差异表达研究  

Study on Gene Differential Expressions of Substance and Energy Metabolism in Chronic Superficial Gastritis Patients of Pi Deficiency Syndrome and of Pi-Wei Hygropyrexia Syndrome

作　　者：杨泽民[1,2] 陈蔚文[1,3] 王颖芳 

机构地区：[1]广州中医药大学脾胃研究所,广州510405 [2]广东药学院基础学院,广州510006 [3]上海中医药大学上海市高校中医内科学E-研究院,上海201203 [4]广尔药学院中药学院,广州510006

出　　处：《中国中西医结合杂志》2012年第9期1180-1187,共8页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金重大研究计划重点项目(No.90209004);广东省自然科学基金重点项目(No.05102323);上海市教育委员会E-研究院建设计划项目(No.E03008)

摘　　要：目的分析慢性浅表性胃炎脾虚证与脾胃湿热证患者体内脂类、蛋白质、核酸、糖类、微量元素和能量代谢情况,从物质能量代谢的角度探讨脾虚证病理发生机制。方法选择2004年6月—2005年3月就诊于广州中医药大学第一附属医院和广东省中医院慢性浅表性胃炎患者8例,其中脾虚证和脾胃湿热证患者各4例,取两组胃黏膜进行DNA芯片实验,利用BRBArrayTools和IPA软件对DNA芯片双通道数据进行数据挖掘和生物信息学分析。结果获得与物质能量代谢相关且表达倍数>2的显著差异表达基因56个,其中上调基因11个,下调基因45个。其中与脂类代谢相关的基因有CRLS1、LRP11、FUT9、GPCPD1、PIGL、SULT1A4、B3GNT1、ST8SIA4和ACADVL,主要涉及脂肪酸、胆固醇、磷脂和糖脂的代谢;与蛋白质代谢相关的基因有ASR-GL1、AARSD1、EBNA1BP2、PUM2、MRPL52、C120RF65、PSMB8、PSME2、UBA7、RNF11、FBXO44、ZFYVE26、CHMP2A、SSR4、SNX4、RAB3B、RABL2A、GOLGA2、KDELR1、PHPT1、ACPP、PTPRF、CRKL、HDAC7、ADPRHL2、B3GNT1、ST8SIA4、DDOST和FUT9,主要涉及到蛋白质的生物合成、蛋白质泛素化、靶向输送和翻译后修饰过程;与核酸代谢相关的基因有DFFB、FLJ35220、TOP2A、SF3A3、CREB3、CRTC2、NR1D2、MED6、GTF2IRD1、C1ORF83、ZNF773和ZMYND11,主要涉及DNA复制与修复,转录调节等;与糖类代谢相关的基因有AGL、B3GNT1、FUT9、ST8SIA4、SULT1A4、DDOST和PIGL,主要涉及糖原的分解和糖复合物的合成;与微量元素代谢相关的基因有COMMD1、SLC39A6、FTL、CHRFAM7A、SCGN和S100A6,主要涉及铜离子、锌离子、铁离子和钙离子代谢;与能量代谢相关的基因有AK3和COX7B,涉及线粒体结构和氧化磷酸化过程。结论脾虚证患者体内脂类、蛋白质、核酸、糖类、微量元素和能量代谢水平明显降低,这可能是导致脾虚证疾病发生的重要机制。Objective To analyze the metabolic levels of energy and substance in chronic superficial gastri- tis （CSG） patients of Pi deficiency syndrome （PDS） and of Pi-Wei hygropyrexia syndrome （PWHS）, including lip- id, protein, nucleic acid, carbohydrate, trace element, and energy metabolism, and to study the pathogenesis mechanism of PDS from substance and energy metabolisms. Methods Recruited were 8 CSG patients who visited at First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine and Guangdong Provirkcial Hos- pital of Traditional Chinese Medicine from June 2004 to March 2005, including 4 patients of PDS and 4 of PWHS. Their gastric mucosae were used for experiments of DNA microarray. The dual-channel DNA microarray data were bioinformatically analyzed by BRB ArrayTools and IPA Software. Results Obtained were fifty-six differentially ex- pressed genes involved in substance and energy metabolisms with the expression fold more than 2, including 11 genes up-regulated and 45 genes down-regulated. Of them, genes correlated to lipid metabolism included CRLS1,LRP11, FUT9, GPCPD1, PIGL, SULT1A4, B3GNT1, ST8SIA4, and ACADVL, mainly involved in the metabolic processes of fatty acid, cholesterol, phospholipids, and glycolipid. Genes correlated to protein metabolism included ASRGL1, AARSD1, EBNA1BP2, PUM2, MRPL52, C120RF65, PSMB8, PSME2, UBA7, RNF11, FBXO44, ZFYVE26, CHMP2A, SSR4, SNX4, RAB3B, RABL2A, GOLGA2, KDELR1, PHPT1, ACPP, PTPRF, CRKL, HDAC7, ADPRHL2, B3GNT1, ST8SIA4, DDOST, and FUT9, mainly involved in the biosynthesis processes of pro- tein, ubiquitination, targeted transport and post-translation modification. Genes correlated to nucleic acid metabo- lism included DFFB, FLJ35220, TOP2A, SF3A3, CREB3, CRTC2, NR1D2, MED6, GTF21RD1, C1ORF83, ZNF773, and ZMYND11, mainly involved in DNA replication and repair, transcription regulation. Genes correlated to carbohydrate metabolism included AGL, B3GNT1, FUT9, ST8SIA4, SULT1A4, DDOST, and PIGL, mainly in- volved in glucogen degradation and g

关 键 词：慢性浅表性胃炎 脾虚证 湿热证 DNA芯片 物质代谢 能量代谢 

分 类 号：R259[医药卫生—中西医结合]