慢性肾功能衰竭中医不同证型患者尿液相关蛋白研究  

Study on Proteins in Urine of Chronic Renal Failure Patients of Different TCM Syndrome Types

作　　者：郝一鸣[1] 洪名超[2] 王文静[3] 金亚明[2] 王忆勤[1] 

机构地区：[1]上海中医药大学中医四诊信息化实验室,上海201203 [2]上海中医药大学附属龙华医院肾病科,上海200032 [3]上海市疾病预防控制中心公共卫生分子生物学实验室,上海200336

出　　处：《中国中西医结合杂志》2012年第9期1196-1199,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：上海市教委科研创新项目(No.08ZZ63);国家中医药管理局中医诊断学重点学科资助项目(No.S30302)

摘　　要：目的研究慢性肾功能衰竭(CRF)中医不同证型患者的尿液蛋白差异。方法选择251例2009年1月—2010年1月上海中医药大学附属龙华医院肾病科住院的CRF患者,其中肝肾阴虚组34例,脾肾气虚组75例,脾肾气阴两虚组56例,脾肾阳虚组32例,阴阳两虚组54例,另选择健康人50名作为对照组。应用SELDI-TOF-MS技术,采用H4蛋白芯片技术进行尿液蛋白质组学研究,用蛋白芯片阅读器PBSⅡ对芯片进行扫描、分析。结果 CRF中医各证型组与对照组间发现49个差异蛋白峰,差异有统计学意义(P<0.01)。各证型组间ROC曲线下面积显示:肝肾阴虚组和脾肾气虚组、脾肾气阴两虚组、脾肾阳虚组、阴阳两虚组以及脾肾气阴两虚组和脾肾阳虚组组间差异度较明显,脾肾气虚组和脾肾气阴两虚组、脾肾阳虚组,脾肾气阴两虚组和阴阳两虚组及脾肾阳虚组和阴阳两虚组差异度一般,脾肾气虚组和阴阳两虚组差异度不明显。结论尿液蛋白标志物能在一定程度上反映CRF各证型组间生物学特点的区别。Objective To study different protein expressions in urine of chronic renal failure （CRF） pa- tients of different Chinese medicine （CM） syndrome types. Methods Recruited were 251 CRF inpatients at the De- partment of Nephrology, Affiliated Longhua Hospital, Shanghai University of Traditional Chinese Medicine from Jan- uary 2009 to January 2010. Of them, there were 34 patients in Gan-Shen yin deficiency group （GSYDG）, 75 in Pi- Shen qi deficiency group （PSQDG）, 56 in Pi-Shen qi-yin deficiency group （PSQYG）, 32 in Pi-Shen yang deficien- cy group （PSYDG）, and 54 in yin-yang deficiency group （YYDG）. Another 50 healthy subjects were recruited as the control group. The proteomic study of the urine was performed with H4 gene chip using surface-enhanced laser desorption/ionization time of flight mass spectrometry （SELDI-TOF-MS）. The gene chips were scanned and ana- lyzed using protein array reader PBSII. Results A total of 49 differential protein peaks were detected between CM syndrome types groups and the control group （P 〈0. 01 ）. The area under the ROC curve of different CM syndrome types showed that obvious difference existed between GSYDG and PSQDG, PSQYG, PSYDG, and YYDG. Obvi- ous difference existed between PSQYD and PSYDG. General difference existed between PSQDG and PSQDG as well as PSYDG. General difference existed between PSQYD and YYDG or between PSYDG and YYDG. No obvious difference existed between PSQDG and YYDG. Conclusion Urine protein biomarkers could reflect different biologi- cal features of CRF patients of different CM syndrome types to some extent.

关 键 词：慢性肾功能衰竭 证型 尿液蛋白 蛋白峰 表面增强激光解析离子化飞行时间质谱 

分 类 号：R277.5[医药卫生—中医学]