黄芪桔梗汤及黄芪黄连汤对克罗恩病大鼠肺与结肠ICAM-1蛋白及mRNA的影响  

Effects of Huangqi Jiegeng Decoction and Huangqi Huanglian Decoction on the Protein and mRNA Expressions of ICAM-1 in the Lung and Colon of Rats with Crohn's Disease

作　　者：杨舒[1] 王新月[1] 杨雪[1] 景姗[1] 朱陵群[2] 王硕仁[2] 

机构地区：[1]北京中医药大学东直门医院消化内科,北京100700 [2]北京中医药大学东直门医院中医内科学教育部重点实验室,北京100700

出　　处：《中国中西医结合杂志》2012年第9期1227-1232,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(No.2009CB522705)

摘　　要：目的观察黄芪桔梗汤及黄芪黄连汤对克罗恩病(CD)大鼠肺与结肠内皮细胞黏附分子-1(inter-cellular adhesion molecule-l,ICAM-1)含量的影响。方法选取56只大鼠采用TNBS水溶液+无水乙醇一次灌肠法建立大鼠CD模型(造模成功率为63%),从造模成功的35只大鼠及正常组大鼠中各随机选出7只作为干预前的模型组及正常组。剩余造模成功的28只大鼠随机分为模型组、西药组(柳氮磺胺吡啶,0.4g/kg)、黄芪桔梗汤组(20.5g/kg)、黄芪黄连汤组(20.8g/kg),每组7只,另选取7只正常大鼠作为正常组,正常组、模型组用等体积纯净水灌胃,每天2次,连续干预3周。于干预前后分别采用Western blot和RT-PCR法测定肺与结肠组织ICAM-1蛋白及mRNA表达。结果与正常组比较,模型组干预前结肠组织ICAM-1蛋白及mRNA水平、肺ICAM-1mRNA均升高(P<0.01,P<0.05),干预后结肠、肺组织ICAM-1蛋白及mRNA水平均升高(P<0.01)。与模型组同期比较各给药组干预后结肠、肺组织ICAM-1蛋白及mRNA水平均降低(P<0.01,P<0.05)。与西药组比较,黄芪桔梗汤组肺组织ICAM-1蛋白表达降低(P<0.05),黄芪黄连汤组结肠组织ICAM-1蛋白表达降低(P<0.05)。结论黄芪桔梗汤、黄芪黄连汤均能下调CD肺及肠组织中过度表达的ICAM-1;黄芪黄连汤在抑制结肠炎性细胞的黏附,减轻局部免疫病理损伤方面效果突出;黄芪桔梗汤减轻肺内炎症和损伤,防止肺纤维化形成方面效果突出。Objective To observe the effects of Huangqi Jiegeng Decoction （HJD） and Huangqi Huangli- an Decoction （HHD） on the intercellular adhesion molecule-I （ ICAM-1 ） content in the lung and colon of rats with Crohn＇s disease （CD）. Methods Totally 56 rats were used to establish the CD rat model using TNBS water so- lution/absolute alcohol enema （with the model successful rate of 63.0% ）. Seven rats randomly selected from 35 successfully modeled rats and from the normal group were recruited as the model group and the normal group be- fore intervention. The rest 28 successfully modeled rats were randomly divided into the model group, the Western medicine group （treated with salazosulfapyridine at 0.4 g/kg）, the HJD group （20.5 g/kg）, and the HHD group （20.8 g/kg）, 7 in each group. Another 7 normal rats were recruited as the normal group. Equal volume of pure water was given to rats in the normal group and the model group by gastrogavage, twice daily, for 3 successive weeks. The protein and mRNA expressions of ICAM-1 in the lung and colon tissues were determined before and after intervention using Western blot and RT-PCR. Results Compared with the normal control group, the protein and mRNA levels of ICAM-1 in the colon tissue, and the mRNA level of ICAM-1 in the lung tissue increased （ P 〈 0.01, P〈0.05）. After intervention the protein and mRNA levels of ICAM-1 in the colon tissue and the lung tissue increased （ P 〈0.01 ） in the model group. Compared with the model group at the same time point, the protein and mRNA levels of ICAM-1 in the colon tissue and the lung tissue decreased in each medication group after interven- tion （P〈0.01, P〈0.05）. Compared with the Western medicine group, the protein level of ICAM-1 in the lung tissue decreased more significantly in the HJD group （P〈0.05）. The protein level of ICAM-1 in the colon tissue also decreased in the HHD group （ P 〈0. 05~）, Conclusions HHD and HJD both could down-regulate the over-ex- pressed ICAM-1 in

关 键 词：克罗恩病 黄芪桔梗汤 黄芪黄连汤 内皮细胞黏附分子-1 肺损伤 

分 类 号：R285.5[医药卫生—中药学]