清心开窍方皂苷对AD大鼠大脑皮层及海马区Bax、Bcl-2、Aβ及βAPP表达的影响  

Effect of Qingxin Kaiqiao Recipe Saponin on the Expressions of Bax,Bcl-2,Aβ,and βAPP in the Cortex and Hippocampus of Alzheimer's Disease Rats

作　　者：谭展望[1] 胡海燕[2] 陈翔[2] 王文花[2] 张晓艳[2] 楚生辉[2] 

机构地区：[1]湖南中医药大学分子病理实验室,湖南长沙410208 [2]温州医学院第二临床学院中医教研室,浙江温州325003

出　　处：《中国中西医结合杂志》2012年第9期1258-1263,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30973780);浙江省自然科学基金资助项目(No.Y2080273)

摘　　要：目的探讨清心开窍方及其皂苷对阿尔茨海默病(Alzheimer's disease,AD)大鼠大脑皮层与海马区Bax蛋白、Bcl-2蛋白、β-淀粉样蛋白(β-amyloid,Aβ)及淀粉样前体蛋白(β-amyloid precursor protein,βAPP)表达的影响。方法选取32只SPF级SD大鼠,将Aβ25-35注入32只大鼠双侧杏仁核制作AD模型,造模后随机分为模型组、盐酸多奈哌齐组(盐酸多奈哌齐片,1.67mg/kg)、清心开窍方组(清心开窍方煎液,12.67mL/kg)及皂苷组(皂苷,6.30mg/kg),每组8只,另选取8只大鼠作为正常组。正常组、模型组给予等量双蒸水灌胃,各组每天干预1次,连续2周。给药结束后进行Morris水迷宫测试,记录各组大鼠水迷宫测试期间第1～5天逃避潜伏期及跨越平台次数。免疫组织化学法检测各组大鼠大脑皮层与海马区Bax、Bcl-2、Aβ及βAPP表达。结果与模型组比较,各用药组大鼠第3～5天逃避潜伏期均缩短,皮层与海马区Bax、Aβ及βAPP表达降低,跨越平台次数增加,皮层Bcl-2表达升高,西药组、中药组海马Bcl-2表达升高,差异均有统计学意义(P<0.01,P<0.05)。与西药组比较,皂苷组βAPP均升高;中药组皮层及海马区Aβ均降低,海马区Bax表达降低,皮层Bcl-2表达升高;皂苷组第3～5天逃避潜伏期明显延长,跨越平台次数减少,皮层及海马区Bcl-2表达降低,差异均有统计学意义(P<0.05,P<0.01)。结论清心开窍方能显著改善AD大鼠学习记忆和空间探索的能力,可能是通过降低大脑皮层与海马区Bax、Aβ、βAPP表达,升高Bcl-2表达而发挥作用。Objective To study the effects of Qingxin Kaiqiao Recipe （QKR） saponin on the expressions of Bax, Bcl-2, 13-amyloid （Aβ）, and 15-amyloid precursor protein （15APP） in the cortex and hippocampus of Alzheimer＇s disease （AD） rats. Methods Thirty-two SD rats of SPF grade were selected. Abeta 25 -35 was in- jected into the bilateral amygdala to prepare the AD model. After modeling rats were randomly divided into the model group, the donepezil hydrochloride group （Donepezil Hydrochloride Tablet, 1.67 mg/kg）, the QLR group （ QLR Decoction, 12.67 mL/kg）, and the saponin group （saponin, 6.30 mg/kg）, 8 rats in each group. Another 8 rats were selected as the normal group. Rats in the normal group and the model group were given with equal volume of double distilled water by gastrogavage. The intervention was performed once daily for 2 successive weeks. The Morris water maze test was carried out by the end of medication. The escape latency and the plat- form crossing times were recorded during the 1 -5 days. The expressions of Bax, Bcl-2, A[3, and 15APP in the cortex and hippocampus were detected using immunohistochemical assay. Results Compared with the model group, the 3rd -5th day escape latency were all shortened in each medication group. The expressions of Bax, A[3, and [3APP decreased in the cortex and hippocampus. The numbers of platform crossing increased. The ex- pression of Bcl-2 in the cortex increased. The expression of Bcl-2 in the hippocampus increased in the donepezil hydrochloride group and the QLR group with statistical difference （ P 〈 0.01, P 〈 0.05）. Compared with the donepezil hydrochloride group, the expression of 15APP increased in the cortex and hippocampus of the saponin group. The expression of Aβ in the cortex and hippocampus decreased, the expression of Bax in the hippocam- pus decreased, and the expression of Bcl-2 in the cortex increased in the QLR group. The escape latency was obviously postponed at day 3 -5, the platform crossing times decreased, the expre

关 键 词：阿尔茨海默病 清心开窍方 细胞凋亡 BAX蛋白 BCL-2蛋白 Β-淀粉样蛋白 

分 类 号：R285.5[医药卫生—中药学]