“百会”透“曲鬓”头针疗法对急性脑出血大鼠脑组织GDNF及VEGF表达的影响  

Effects of the Scalp Acupuncture at Baihui(DU20) through Qubin(GB7) on the Expressions of GDNF VEGF in the Brain Tissue of Rats with Acute Intracerebral Hemorrhage

作　　者：张国威[1] 邹伟[2] 刘芳[3] 郭新年 赵佳辉[5] 孙晓伟[2] 

机构地区：[1]内蒙古民族大学护理学院公共卫生学教研室,内蒙古通辽028043 [2]黑龙江中医药大学附属第一医院神经内科,哈尔滨150040 [3]黑龙江省哈尔滨市第一医院干部病房,哈尔滨150010 [4]黑龙江省康复医院针灸科,哈尔滨150018 [5]黑龙江省中医研究院针灸科,哈尔滨150036

出　　处：《中国中西医结合杂志》2012年第9期1264-1270,共7页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30772840)

摘　　要：目的观察"百会"透"曲鬓"头针疗法对急性脑出血大鼠脑组织胶质源性神经营养因子(GDNF)和血管内皮生长因子(VEGF)表达的影响。方法选取健康雄性Wistar大鼠150只制备脑出血模型,随机分为模型组、针刺组、西药组,每组50只;每组再随机分为6h、1d、2d、3d、7d五个亚组,每个亚组10只;另设10只正常大鼠作为空白组。针刺组捆绑固定,针刺患侧"百会"透"曲鬓"穴。西药组给予茴拉西坦稀释液1mL灌胃,3次/d。模型组大鼠给予针刺组相同捆绑30min/d,生理盐水1mL灌胃,3次/d。运用原位杂交及免疫组化法检测各组大鼠脑组织中GDNF和VEGF阳性细胞的表达。结果与空白组比较,模型组6h～3dGDNF阳性细胞数增多,各时间点VEGF阳性细胞数增多,差异均有统计学意义(P<0.01)。针刺组各时间点GDNF阳性细胞数均较模型组和西药组增多,差异均有统计学意义(P<0.01);西药组各时间点与模型组比较,差异均无统计学意义(P>0.05)。与模型组比较,针刺组6h～3dVEGF阳性细胞数减少,7d时间点VEGF阳性细胞数增多,且高于西药组同期,差异均有统计学意义(P<0.01)。结论 "百会"透"曲鬓"头针疗法可能在脑出血急性期通过促进内源性GDNF表达、早期抑制VEGF的表达并后期促进VEGF的表达等途径发挥神经重塑作用。该方法可能在脑出血急性期具有良性的双向调节作用,疗效优于茴拉西坦。Objective To observe the effects of the scalp acupuncture at Baihui （DU20~） through Qubin GB7） on the expressions of glial cell line-derived neutrotrophic factor （GDNF） and vascular endothelial growth factor （VEGF） in the brain tissue of rats with acute intracerebral hemorrhage （AICH）. Methods Totally 150 healthy male Wistar rats were used to prepare the intracerebral hemorrhage （ICH） model. They were randomly divided into the model group, the acupuncture group, and the Western medicine group, 50 in each group. Rats in each group were then randomly divided into five subgroups, i. e., 6 h, day 1, day 2, day 3, and day 7, 10 in each subgroup. Another 10 normal rats were recruited as the blank control group. Rats in the acupuncture group were acupunctured at Baihui （DU20） through Qubin （GB7） on the lesion side. Rats in the Western medicine group were administered with aniracetam diluent 1 mL by gastrogavage, 3 times daily. Rats in the model group were bound the same way as those in the acupuncture group 30 min daily, and then administered with normal sa- line 1 mL by gastrogavage, 3 times daily. The expressions of GDNF and VEGF in the brain tissue of the rats in each group were detected using in situ hybridization and immunohistochemical methods. Results Compared with the blank control group, the number of GDNF positive cells in the model group increased at 6 h-3 days, and the number of VEGF positive cells in the model group increased at each time point, showing statistical difference （ P〈0.01 ）. The numbers of GDNF positive cells increased in the acupuncture group at each time point, showing statistical difference when compared with those of the model group and the Western medicine group （P 〈0.01 ）. There was no statistical difference between the model group and the Western medicine group at each time point （P〉0.05）. Compared with the model group, the number of VEGF positive cells in the acupuncture group de- creased at 6 h -3 days. The VEGF positive cells increased

关 键 词：“百会”透“曲鬓”头针疗法 脑出血 神经重塑 胶质源性神经营养因子 血管内皮生长因子 

分 类 号：R245[医药卫生—针灸推拿学]