肾衰Ⅱ号方对慢性肾功能衰竭大鼠肾组织AngⅡ及nNOS蛋白表达的影响  

Effects of Shenshuai Ⅱ Recipe on the Protein Expressions of Ang Ⅱ and nNOS in Chronic Renal Failure Rats' Nephridial Tissue

作　　者：秦军燕[1] 王琛[1,2] 邵命海[1] 杨婧[1] 李睿琳[1] 

机构地区：[1]上海中医药大学附属曙光医院肾病科,上海200021 [2]上海中医药大学上海市教委创新团队,肝肾疾病病证教育部重点实验室,上海市中医药重点实验室,上海200021

出　　处：《中国中西医结合杂志》2012年第9期1280-1284,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30973723);上海市科委基金资助项目(No.08411962400)

摘　　要：目的观察肾衰Ⅱ号方对5/6(ablation/infarction,A/I)肾切除慢性肾功能衰竭(chronic renal failure,CRF)大鼠残余肾组织纤维化及血管紧张素Ⅱ(AngⅡ)和神经型一氧化氮合酶(nNOS)表达的影响,并初步探讨其作用机制。方法选取57只SD雄性大鼠按5/6(A/I)肾衰模型法制备大鼠模型。造模后随机分为模型组、肾衰Ⅱ号方组(中药组,肾衰Ⅱ号方浓煎液2mL灌胃)和西药组(氯沙坦钾合福辛普利钠混悬液,2mL灌胃),每组15只,另选取15只大鼠作为正常组,正常组和模型组予等量纯净水灌胃,各组均每天干预1次,连续干预60天。检测各组大鼠SCr、BUN、内生肌酐清除率(creatinine clearance rate,CCr),采用Western blot法测定大鼠残余肾皮质、髓质AngⅡ和nNOS蛋白表达,观察肾组织病理形态。结果与正常组比较,模型组SCr、BUN升高,CCr降低(P<0.01),提示造模成功。与本组干预前比较,中药组及西药组SCr、BUN水平下降,CCr水平升高(P<0.01)。与模型组比较,中药组及西药组SCr、BUN水平及髓质AngⅡ表达均降低(P<0.01,P<0.05),CCr水平及皮质、髓质nNOS表达明显升高(P<0.01,P<0.05)。肾组织病理显示,中药组病理变化明显减轻,优于模型组。结论肾衰Ⅱ号方可以改善5/6肾切除CRF大鼠的肾功能,减轻肾间质纤维化,其作用机制可能与调节肾内失衡的AngⅡ和nNOS信号转导途径有关。Objective To observe the effects of Shenshuai Ⅱ Recipe （SSR） on the fibrosis of remnant nephridial tissue and expressions of Ang Ⅱ and nNOS in the chronic renal failure （CRF） rats induced by 5/6 ab- lation/infarction （A/I）, and to preliminarily investigate its mechanism of action. Methods Fifty-seven SD male rats were used to prepare the CRF rat model by means of 5/6 A/I. After modeling, they were randomly divided into the model group, the SSR group （2 mL SSR condensed decoction given by gastrogavage）, and the Western medicine group （treated by 2 mL suspension of Iosartan potassium and fosinopril sodium given by gastrogav- age）, 15 rats in each group. Another 15 rats were recruited as the normal control group. Equal volume of pure water was given to rats in the normal control group and the model group. Relevant treatment was given to rats in each group once daily, for 60 successive days. The serum creatinine （SCr）, blood urea nitrogen （BUN）, and creatinine clearance rate （CCr） were detected. The expressions of angiotensin Ⅱ （Ang Ⅱ ） and nervous system type nitric oxide synthase （nNOS） in the remnant renal cortex and the medulla were detected by Western blot. The pathomorphology of the nephridial tissue was observed. Results Compared with the normal control group, the levels of SCr and BUN increased （ P〈0.01 ） and the level of CCr decreased （ P〈0.01 ） in the model group, indicating a successful modeling. Compared with the same group before treatment, the levels of SCr and BUN decreased and the level of CCr increased in the SSR group and the Western medicine group （ all P 〈 0. 01 ）. Compared with the model group after treatment, the levels of SCr and BUN decreased, and the expression of Ang Ⅱ in the medulla decreased in the SSR group and the Western medicine group （P〈0. 01, P〈0.05）. The levels of CCr and protein expressions of nNOS in the renal cortex and the medulla obviously increased in the SSR group and the Western medicine group （ P�

关 键 词：肾衰Ⅱ号方 血管紧张素Ⅱ 神经型一氧化氮合酶 肾间质纤维化 

分 类 号：R285.5[医药卫生—中药学]