水稻(Oryza sativa L.)苹果酸酶(OsNADP-ME3)基因在逆境下的表达特性研究  

Expression Characteristics of Rice(Oryza sativa L.) Malic Enzyme(OsNADP-ME3) Gene under Environmental Stress

作　　者：李秀峰 张欣欣[1] 高野哲夫[2] 柳参奎[1] 

机构地区：[1]东北油田盐碱植被恢复与重建教育部重点实验室东北林业大学盐碱地生物资源环境研究中心,哈尔滨150040 [2]东京大学亚洲生物资源环境研究中心,东京1880002

出　　处：《基因组学与应用生物学》2012年第4期327-332,共6页Genomics and Applied Biology

基　　金：国家林业局948项目(2008-4-29);黑龙江省杰出青年科学基金(JC200609)共同资助

摘　　要：水稻苹果酸酶(NADP-ME)是多基因家族,由3个胞质型NADP-ME和1个质体型NADP-ME构成。本研究针对水稻胞质型成员(命名为NADP-ME3)(NM_001061367)进行初步的功能解析。克隆获得的NADP-ME3基因的cDNA序列全长为2240bp,其中5'非翻译区为151bp,3'非翻译区为376bp,开放读码框(ORF)长1713bp,编码570个氨基酸。为研究NADP-ME3在逆境胁迫下的表达量变化,Northern blot检测结果显示,在NaCl、NaHCO3和PEG胁迫条件下,NADP-ME3随胁迫处理时间的不同表达量呈现不同程度的变化,推断NADP-ME3可能与非生物胁迫有应答关系,将NADP-ME3转入拟南芥中并通过观察转基因拟南芥在非生物胁迫下表型变化,发现NADP-ME3能够在一定程度上提高植物对非生物胁迫的耐受性。Rice NADP-MEs are a multigene family of three cytoplasmic NADP-MEs and one plastidic NADP-ME. In this study, we focus on the expression characteristics of one of the cytoplasmic members （named as NADP-ME3） （NM_001061367）. The cDNA sequence of the cloned NA DP-ME3 is 2 240 bp, and the 5＇ untransla- ted region （UTR） is 151 bp while the 3＇ UTR is 376 bp. The open reading frame is 1 712 bp, encoding 570 amino acids. The expressions of NA DP-ME3 mRNA under a variety of abiotic stresses were detected with Northern blot. The results indicated at the exposure to NaC1, NaHCO3 and PEG, the expression level of NA DP-ME3 changed with the different lengths of exposure duration. Therefore, the NADP-ME3 may be involved in response to abiotic stresses. Then, ？CA DP-ME3 was overexpressed in Arabidopsis plants to observe the changes of phenotypes, and it was found that ？CA DP-ME3 could improve plant resistance to abiotic stresses to a certain extent.

关 键 词：水稻 NADP-ME3 基因表达 转基因植物 

分 类 号：S511[农业科学—作物学]