骨向分化骨髓间充质干细胞的免疫学特性研究  

Immunologic properties of osteogenic differentiated bone mesenchymal stem cells

作　　者：方军[1] 李华壮[1] 丁刚[2] 赵光宗[1] 高克海[1] 陈景春[1] 

机构地区：[1]潍坊医学院青州临床学院骨科,青州262500 [2]潍坊医学院青州临床学院口腔科,青州262500

出　　处：《中华创伤杂志》2012年第9期838-841,共4页Chinese Journal of Trauma

基　　金：国家自然科学基金资助项目（81070799）;山东省优秀中青年科学家科研奖励基金资助项目（BS2010SW033）

摘　　要：目的探讨骨向分化骨髓问充质干细胞（bonemesenchymalstemcells，BMSCs）的免疫学特性。方法正常成人骨髓分离的BMSCs在骨向诱导培养基中培养2周后，将未分化的BMSCs和骨向分化的BMSCs与同种异体T淋巴细胞、植物血凝素（PHA）共培养，MrITr法检测T淋巴细胞的增殖情况；ELISA法测定骨向分化2周的BMSCs上清以及共同反应5d的混合淋巴细胞反应（mixedlymphocytesreaction，MLR）上清转化生长因子（transforminggrowthfactor-β1，TGF—β1）的浓度，并通过加入抗TGF—β抗体检测MLR情况。结果无论是未分化的BMSCs还是骨向分化的BMSCs都不会引起同种异体T淋巴细胞的增殖，并且都能抑制PHA诱导的T淋巴细胞增殖。MLR中的TGF-β1浓度显著升高。抗TGF—β抗体能够抵消骨向分化的BMSCs免疫抑制作用。结论骨向分化的BMSCs具有低免疫原性和免疫抑制特性。Objective To investigate the immunologic properties of osteogenic differentiated bone mesenchymal stem ceils （BMSCs）. Methods BMSCs were isolated from normal volunteers and in- duced in osteogenic medium for two weeks. Then, non-differentiated/osteogenic differentiated BMSCs were co-cultured with allogenic T cells and phytohemagglutinin （PHA）. The proliferation of T cells was examined by MTY method. The concentrations of TGF-β1 in osteogenic differentiated BMSCs supernatants at week 2 and mixed lymphocytes reaction （MLR） supernatants at day 5 were determined by ELISA. Al- so, anti-TGF-β antibody was added into the MLR to detect the response of the mixed T cells. Results Non-differentiated and osteogenic differentiated BMSCs did not induce proliferation of the allogeneic T cells but both suppressed the proliferation of the T cells mediated by PHA. The TGF-β1 concentrations had significant elevation in the MLR. Anti-TGF-β antibody could counteract the immunosuppressive func- tion of the osteogenic differentiated BMSCs. Conclusion Osteogenic differentiated BMSCs possess low immunogenicity and immunosuppressive property.

关 键 词：骨髓 间质干细胞 细胞分化 免疫抑制 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]