三氯乙烯致敏豚鼠体液免疫变化  

Changes in humoral immunity in sensitized guinea pigs exposed to trichloroethylene

作　　者：朱启星[1,2] 徐辉 冷静[4] 沈彤[2] 

机构地区：[1]安徽医科大学公共卫生学院劳动卫生与环境卫生系,合肥230032 [2]安徽医科大学公共卫生学院皮肤病研究所,合肥230032 [3]安徽省肿瘤防治所 [4]安徽省医学科学研究院

出　　处：《中华劳动卫生职业病杂志》2012年第9期641-644,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目（30872147）;教育部科学技术研究重点项目（210100）;安徽省自然科学基金项目（11040606M213）;安徽高校省级自然科学研究重点项目（KJ2009A73）

摘　　要：目的探讨三氯乙烯（TCE）致敏豚鼠血清中补体（C）和免疫球蛋白（Ig）的变化。方法选用体重250～300g的雌性豚鼠36只，随机分成空白对照组（5只），溶剂（橄榄油）对照组（5只），TCE实验组（26只）。根据豚鼠最大值试验（GPMT）方法对豚鼠进行染毒。激发接触后24h观察和记录豚鼠背部受试区的皮肤反应情况，进行评分。按皮肤致敏反应积分将TCE处理组豚鼠分为致敏组（积分≥1）和未致敏组（积分为0）。分别在实验结束后24、72h采血，检测血清中C3、C4、IgA、IgG、IgM浓度。结果TCE处理组豚鼠致敏率为65．38％（17／26）。TCE致敏24、72h组C3含量[24h：（99．75±1．45）μg／ml，72h：（93．28±3．61）μg／ml]均明显低于溶剂对照组【（112．30±9．10）μg／ml】，差异均有统计学意义（P〈0．05）；与溶剂对照组比较，致敏24、72h组C4含量[24h：（34．63±2．53）μg／ml，72h：（33．82±2．76）μg／ml]均明显低于溶剂对照组[（43．87±3．65）μg／ml]，差异均有统计学意义（P〈0．05）。TCE致敏24、72h组、TCE未致敏24、72h组IgA、IgM含量均明显低于溶剂对照组，差异均有统计学意义（P〈0．05）。与TCE未致敏组比较，TCE致敏24、72h组血清中IgA水平均降低，差异有统计学意义（P〈0．05）。各组血清中IgG的含量无明显改变。结论TCE致敏豚鼠皮肤后，血清中C3、C4含量降低，体液免疫功能发生紊乱。Objective To study the changes of serum complement C and immunoglobulin （Ig） in sensitized guinea pigs exposed to trichloroethylene. Methods Thirty six white female guinea pigs （250-300 g） were randomly divided into blank control group （5 guinea pig）, solvent （olive oil） control group （5 guinea pig） and TCE treatment group （26 guinea pig）. According to guinea pig maximization test （GPMT）, guinea pigs were exposed to TCE. After stimulating contact for 24 h, the skin reactions of guinea pig back test area were recorded and scored. According to Skin sensitization integral, the guinea pigs treated with TCE were divided into the sensitized group （score≥ 1 ） and un-sensitized group （score 0）. The concentrations of serum C3, C4, IgA, IgG and IgM were detected in 24 and 72 h, respectively after the experiment. Results The sensitization rates of group treated by TCE was 65.38%. The serum C3 levels of groups sensitized to TCE for 24 and 73 h were 99.75±1.45 and 93.28±3.61g/ml, respectively, which were significantly lower than that （ 112.30±9.10 g/ml） of solvent control group （P〈0.05）. Also The serum C4 levels of groups sensitized to TCE for 24 and 73 h were 34.63±2.53 and 33.82±2.76g/ml, respectively, which were significantly lower than that （43.87±3.65 g/ml） of solvent control group （P〈0.05）. The serum IgA and IgM levels of groups sensitized to TCE and unsensitized to TCE for 24 and 72 h were significantly lower than those of solvent group （P〈0.05）. as compared with unsensitized groups, the serum IgA levels of the groups sensitized to TCE for 24 and 72 h significantly decreased （P〈0.05）. Conclusion After the guinea pig skin was sensitized to TCE, the serum C3, C4 levels decreased, the immune function disordered.

关 键 词：三氯乙烯 豚鼠 补体 免疫球蛋白类 

分 类 号：R114[医药卫生—卫生毒理学]