骨髓间充质干细胞植入对急性百草枯染毒大鼠肺损伤的影响  

Optimal conditions of bone marrow mesenchymal stem cells on paraquat-induced acute lung injury in rats

作　　者：黄杨[1] 尹文[1] 张秀敏[2] 王玉同[1] 余厚友[1] 郝璐[1] 刘健[1] 

机构地区：[1]第四军医大学西京医院急诊科,西安710033 [2]第四军医大学西京医院病理科,西安710033

出　　处：《中华劳动卫生职业病杂志》2012年第9期645-649,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家青年科学基金资助项目（81101413）;陕西省自然科学基金资助项目（2010JM4051）

摘　　要：目的探讨骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSCs）的植入对急性百草枯（paraqua，PQ）染毒大鼠肺损伤的影响及最佳植入条件。方法200只雌性大鼠随机分为6组。PQ组（15只）：腹腔注射质量浓度为20％的PQ溶液（15ms／kg）；BMSCs治疗1组：PQ染毒后6h分别经尾静脉注射1×10^5、1×10^6、1×10^7、1×10^8个BMSCs（1ml／只），每剂量组15只；BMSCs治疗2组：分别于PQ染毒后1、6、12、24h经尾静脉注射BMSCs 1×10^7个／只（1ml），每剂量组15只；BMSCs对照1组：经尾静脉注射1×10^5、1×10^6、1×10^7、1×10^8个BMSCs（1ml／只），每剂量组15只；BMSCs对照2组（15只）：尾静脉注射1×10^7个BMSCs（1ml／只），正常对照组（5只）：大鼠腹腔注射等容积生理盐水，观察不同处理后1、3、7d各组大鼠肺湿／干比、血浆中肿瘤坏死因子（TNF-α）和丙二醛（MDA）水平的变化。结果PQ组大鼠染毒后3、7d肺湿／干比，染毒后1、3、7d血浆中TNF—α和MDA含量均明显高于正常对照组，差异有统计学意义（P〈0．01）。BMSCs治疗1组注射1×10^6、1×10^7个BMSCs后7d的肺湿，干比均明显低于PQ组，BMSCs治疗1组注射1×10^5、1×10^6、1×10^7个BMSCs后3、7d血浆中TNF-α含量均明显低于PQ组，BMSCs治疗1组注射1×10^6、1×10^7个BMSCs后3d、注射1×10^5、1×10^6、1×10^7个BMSCs后7d血浆中MDA含量明显低于PQ组，差异均有统计学意义（P〈0．05或P〈0．01）。BMSCs治疗2组在染毒后1、6、12h注射BMSCs后7d，肺湿／干比明显低于PQ组，BMSCs治疗2组染毒后1、6、12h注射BMSCs后3、7d血浆中TNF-α含量明显低于PQ组，BMSCs治疗2组在染毒后6h注射BMSCs后3d、染毒后1、6、12h注射BMSCs后7d血浆中MDA含量明显低于PQ组，差异均有统计学意义（P〈0．01）。结论BMSCs对PQ染毒大鼠肺损伤有保护作用，其效果与BMSCs的植入时间和数量有密切关系。染毒后6h、静脉注射1×10Objective To investigate the effect of bone marrow mesenchymal stem cell （BMSC） transplantation on acute lung injury induced by paraquat （PQ） poisoning in rats, and to identify the optimal transplantation conditions. Methods Two hundred female rats were randomly divided into six groups, i.e., PQ group （n=15）, BMSC treatment group 1 （n=15 for each subgroup）, BMSC treatment group 2 （n=15 for each subgroup）, BMSC control group 1 （n=15 for each subgroup）, BMSC control group 2 （n=15）, and normal control group （n=5）. In the PQ group, each rat was intraperitoneally injected with 20% PQ solution （15 mg/kg）. In BMSC treatment group 1, each rat was injected via the caudal vein with 1×10^5, 10^6, 10^7 or 10s BMSCs （1 ml） after 6 hours of PQ exposure. In BMSC treatment group 2, each rat was injected via the caudal vein with 1×10^7 BMSCs （ 1 ml） after 1, 6, 12, or 24 hours of PQ exposure. In BMSC control group 1, each rat was injected via the caudal vein with 1×10^5, 10^6, 10^7, or 10s BMSCs （1 ml）. In BMSC control group 2, each rat was injected via the caudal vein with 1×107 BMSCs （1 ml）. In the normal control group, each rat was intraperitoneally injected with an equal volume of 0.9% saline. The lung wet/dry weight ratio and plasma tumor necrosis factor-α （TNF-α） and malondialdehyde （MDA） levels were measured at 1, 3, and 7 days after various treatments. Results Compared with the normal control group, the PQ group showed significantly higher lung wet/dry weight ratios at 3 and 7 days after PQ exposure and significantly higher plasma TNF-α and MDA levels at 1, 3, and 7 days after PQ exposure （P〈0.01）. Compared with the PQ group, BMSC treatment group 1 showed significantly lower lung wet/dry weight ratios at 7 days after injection of 1×10^6 and 10^7 BMSCs, significantly lower plasma TNF-α levels at 3 and 7 days after injection of 1×10^5, 10^6, and 10^7 BMSCs, and significantly lower plasma MDA levels at 3 clays after injeetion of 1×10^6 and 10^7 BMSC

关 键 词：百草枯 肺损伤 造血干细胞移植 

分 类 号：R114[医药卫生—卫生毒理学]