1，3-丁二烯作业工人尿中生物标志物的研究  

Study on urine biomarkers in 1, 3-butadiene exposed workers

作　　者：程学美[1,2] 焦燕妮[2] 陈金东[2] 单宝德[2] 夏昭林[1] 

机构地区：[1]复旦大学公共卫生学院,上海200032 [2]山东省疾病预防控制中心

出　　处：《中华劳动卫生职业病杂志》2012年第9期661-666,共6页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金（NSFC81072280）;山东省医药卫生研究项目（2009HZ050）;973项目（2011CB503801）;上海市卫生局课题（08GWD12）;山东省自然科学基金-青年基金项目（ZR2010HQ042）

摘　　要：目的探讨1，3-丁二烯（BD）接触工人的内剂量生物标志物，为BD接触工人生物限值的制定提供基础依据。方法选择某橡胶厂BD接触工人44名作为接触组，不接触BD的25人为对照组（同一企业锅炉车间工人12名，为内对照；某机构文员13名，为外对照）。收集班末尿，用固相微萃取柱对尿液进行处理，UPLC—MS／MS检测，同位素内标定量尿液中BD代谢产物3，4-二羟基硫尿酸（DHBMA）和1-羟甲基-2-苯硫酚尿酸（MHBMA）的浓度，同时用气相色谱-火焰离子化检测器测定工人作业场所空气中BD浓度，比较其内外暴露指标间的相关性。尿中DHBMA和MHBMA及空气中BD测定数据均经对数转换后统计分析。结果接触组尿中DHBMA[（2．51±0．44）μg／L]和（MHBMA±DHBMA）[（2．68±0．27）μg/L]明显高于对照组[DHBMA：（2．20±0．25）μg／L，（MHBMA±DHBMA）：（2．49±0．34）μg/L]，差异有统计学意义（P〈0．01）；尿中DHBMA的含量受空气中BD浓度影响明显（r=0．539，P=0．001），回归方程为y=2．417±0．520x，模型的调整R^2为0．262；尿中MHBMA不受到吸烟、饮酒和工龄的影响。结论在职业环境暴露水平下，班末尿中DHBMA可以作为BD接触的内剂量标志物。Objective To discuss the urine biomarkers in 1,3-butadiene exposed workers, and to provide basement for establishing biological limit value. Methods 44 BD exposed workers as exposure group and 25 BD non-exposed people as control group including 12 workers in boiler workshop in the same factory and 13 people in one public institute, we collected their in-end-of shift urine, then detected urine BD-derived mercapturic metabolites [3, 4-dihydroxybutyl mercapturic acid （DHBMA）, 1-and 2-monohydroxy-3-butenyl mercapturic acid （MHBMA）] concentrations using UPLC-MS/MS method. Meanwhile, we detected air BD concentration with GC-FID in the workplace, and compared their relationship. Results lgDHBMA and lg （MHBMA±DHBMA） levels in exposed group （lgDHBMA： （2.51±0.44） μg/L, 1g[MHBMA±DHBMA： （2.68± 0.27） μg/L] were higher than which in control group （lgDHBMA： （2.20±0.25） μg/L, 1g （MHBMA ±DHBMA： （2.49±0.34） μg/L）, and the differences were significant （P〈0.01）. Urine DHBMA was obviously influenced by air BD concentrations （r=0.539, P=-0.001 ）. The equation of Multiple Regression Analysis was y=2.417±0.520x （x represents air BD dose, and y represents urinary DHBMA level）. Adjusted R^2 of this model was 0.262. Urinary MHBMA was not affected by smoking, alcohol and years of works. Conclusion Urine metabolite DHBMA in BD-exposed workers might be major biological exposure indice.

关 键 词：1 3-丁二烯类 生物学标记 色谱法 高压液相 

分 类 号：R1[医药卫生—公共卫生与预防医学]