苯胺经皮肤吸收规律及其防护研究  

Study of anilin e＇s penetration capability through rats＇ complete skin in vitro and normal lab gloves

作　　者：冯峰[1] 何百寅[1] 叶雪兰 刘常青[1] 谢友良[1,3] 赖小平[1,3] 周应军[4] 

机构地区：[1]广州中医药大学新药研究开发中心,510006 [2]广州潘高寿药业股份有限公司 [3]中医药数理工程研究院 [4]中南大学药学院

出　　处：《中华劳动卫生职业病杂志》2012年第9期683-685,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：东莞市科技计划重点项目（201010810203）

摘　　要：目的研究苯胺对完整小鼠腹皮的透过性和2种实验室常用手套对苯胺的防护作用。方法采用Franz扩散池，进行0．102mg／ml和0．010mg／ml的苯胺乙醇溶液体外经皮肤吸收试验和手套透过试验，以完整小鼠腹皮、手套为渗透屏障，高效液相色谱一二极管阵列检测器法测定苯胺的透过率。结果2种浓度苯胺12h内透皮过程呈零级动力学规律，24h累积透过率分别为51．71％和48．31％；吸收液中苯胺浓度至少为18μg，L，医用一次性乳胶手套无法阻止0．010mg／ml的苯胺渗透，天然乳胶工业手套可阻止渗透。结论0．102和0．010mg／ml的苯胺乙醇溶液12h内透过小鼠腹皮呈零级动力学规律；医用一次性乳胶手套不能完全阻止0．010mg／ml的苯胺渗透，而天然乳胶工业手套可以阻止苯胺渗透。Objective To study the permeability of intact mouse abdominal skin to aniline and the protective capability of two typical lab gloves against aniline. Methods A Franz diffusion cell was used to perform in vitro transdermal absorption test and glove permeation test for aniline （0.102 mg/ml and 0.010 mg/ml）. The permeabilities of intact mouse abdominal skin and gloves to aniline were measured by high performance liquid chromatography-diode array detection. Results The transdermal penetration of the two concentrations of aniline followed zero order kinetics within 12 h, exhibiting total aniline permeabilities within 24 h of 51.71% and 48.31%, respectively. The absorption liquid had an aniline concentration of at least 18 p, gfL. The medical disposable latex glove could not stop the penetration of 0.010 mg/ml aniline, but the industrial natural latex glove could. Conclusion The penetration of 0.102 mg/ml and 0.010 mg/ml aniline through the mouse abdominal skin follows zero order kinetics within 12 h. The medical disposable latex glove cannot stop the penetration of 0.010 mg/ml aniline, but the industrial natural latex glove can.

关 键 词：苯胺化合物 透皮肤吸收 手套 防护 

分 类 号：R131[医药卫生—劳动卫生]