17aα-D-高炔雌二醇-3-乙酯对荷瘤小鼠抑瘤作用的实验研究  

Inhibitive Effects of 17act-D-homo Ethynylestradiol-3-acetate in Tumor-bearing Mice

作　　者：韩英[1] 宋娜玲[1] 王月英[1] 孙元明[1] 吴红英[1] 李德冠[1] 

机构地区：[1]中国医学科学院北京协和医学院放射医学研究所,天津市分子核医学重点实验室,天津300192

出　　处：《中国辐射卫生》2012年第3期266-267,共2页Chinese Journal of Radiological Health

基　　金：国家973重大专项(2011CB964800-G);国家自然科学基金(81072237;30970867);天津市自然科学基金重点项目(11JCZDJC19100)

摘　　要：目的观察17aα-D-高炔雌二醇-3-乙酯及联合137Csγ射线对白血病L1210的抑制作用。方法通过肿瘤细胞悬液接种法,制备IRM-1小鼠L1210白血病动物模型,实验分对照组、单放组、17aα-D-高炔雌二醇-3-乙酯(低、中、高)药物组及药物合用照射组,采用腹腔注射给药法,连续7d,1次/d,末次给药第5天处死动物,分别检测肿瘤抑制率、骨髓有核细胞数、胸腺与脾重指数指标,比较各组间的差别。结果 17aα-D-高炔雌二醇-3-乙酯各组瘤重明显小于对照组,合用照射组对荷瘤小鼠L1210白血病有较强抑制作用。结论 17aα-D-高炔雌二醇-3-乙酯对L1210白血病有明显的抑瘤作用及保护小鼠的免疫器官的作用,与γ射线合用时,能提高抑瘤疗效。Objective To observe the inhibitory effect of 17aα - D - homo ethynylestradiol - 3 - acetate combined 137Cs radiation in leukemia L1210 cells. Methods The animal model of leukemia （Ll210） was prepared by tumor cell inoculation. All animal divided into six groups, control group, group treated only with radiation, 17act -D -homo ethynylestradiol - 3 - acetate high - level group, 17aα - D - homo ethynylestradiol - 3 - acetate mid - level group, 17aα - D - homo ethy- nylestradiol -3 -acetate low -level group and group combined E838 and radiation. Mice with leukemia （L1210） followed intraperitoneal injection of 17aα - D - homo ethynylestradiol - 3 - acetate once a day for 7 days. Tumor inhibition rate, the number of bone marrow nucleated cells, thymus index and spleen index were detected at the fifth day after the last administration and compare the difference in different groups. Results Tumor in every groups treated with 17aα - D - homo ethy- nylestradiol - 3 - acetate significant smaller than the control. 17aα - D - homo ethynylestradiol - 3 - acetate combined radation has great inhibition effect in tumor - bearing mice （L1210）. Conclusion 17act - D - homo ethynylestradiol - 3 - acetate has significant inhibition effect in leukemia （L1210） and could protect the immune organs in mice. 17aα- D -homo ethy- nylestradiol - 3 - acetate combined radiation could enhance the anti - tumor therapy.

关 键 词：17aα-D-高炔雌二醇-3-乙酯 恶性肿瘤 放射治疗 IRM-1小鼠 

分 类 号：R815[医药卫生—放射医学]