呼吸道白念珠菌分离株生物膜表型差异的机制  

Mechanism of phenotype difference in biofilm formation by Candida albicans isolated from the respiratory tract

作　　者：阳隽[1] 张天托[2] 朱家馨[2] 

机构地区：[1]广州医学院第二附属医院呼吸内科,广东广州510260 [2]中山大学第三附属医院呼吸内科,广东广州510630

出　　处：《南方医科大学学报》2012年第9期1307-1311,共5页Journal of Southern Medical University

基　　金：广州市医药卫生科技一般引导项目(2008-YB-161)

摘　　要：目的探讨呼吸道白念珠菌分离株生物膜形成能力存在差异的可能分子机制。方法呼吸道白念珠菌临床分离株及标准株ATCC90028体外粘附生长24 h形成生物膜,用XTT减低法测定其增殖情况,并分别提取生物膜态白念珠菌总RNA,用荧光定量RT-PCR的方法测定转录因子CPH1、EFG1和粘附分子ALS3、HWP1基因的表达,采用△Ct的方法计算其相对表达量。结果根据粘附生长的白念珠菌增殖情况,有8株临床株形成生物膜能力"高",另7株临床株及标准株ATCC90028形成生物膜能力"低"。生物膜表型差异的菌株间转录因子CPH1、EFG1的表达无显著差异(P>0.05),而粘附分子ALS3、HWP1基因的表达有显著差异(P<0.05)。结论呼吸道白念珠菌分离株生物膜形成存在表型差异,其差异的机制可能与转录因子CPH1和EFG1以外的基因调控相关。Objective To explore the molecular mechanism of the differences in biofilm formation abilities of Candida albicans isolated from the respiratory tract. Methods The biofilms formed by Candida albicans isolates from the respiratory tract and the standard strain ATCC90028 were examined for bacterial proliferation using XTT reduction assay. The mRNA expression of CPH1, EFG1, ALS3 and HWP1 in the isolates were measured with fluorescent quantitative RT-PCR. Results XTT reduction assay demonstrated a strong ability of biofilm formation in 8 clinical isolates, and a relatively low biofilm formation ability in 7 clinical isolates and ATCC90028 strain. The strong and weak biofilm formers showed significant differences in ALS3 and HWP1 mRNA expressions （P〈0.05） but not in EFG1 or CPH1 mRNA expressions （P〉0.05）. Conclusion The clinical isolates from the respiratory tract have different biofilm formation abilities under regulation by genes other than the transcription factors CPH1 and EFG1.

关 键 词：白念珠菌 生物膜 转录因子 

分 类 号：R379[医药卫生—病原生物学]