Photoelectrochemical Detection of Glucose by Using an Enzyme-Modified Photoelectrode  

作　　者：赵常志 张兆霞 赵影 俞佳 

机构地区：[1]College of Chemistry&Molecular Engineering,Qingdao University of Science&Technology,Qingdao,Shandong 266042,China

出　　处：《Chinese Journal of Chemistry》2012年第8期1851-1856,共6页中国化学（英文版）

摘　　要：A new photoelectrochemical method for the determination of glucose based on the photoelectrochemical effect of poly（thionine） photoelectrode to hydrogen peroxide （H202） was reported. The H2Oz-sensitive photoelectrode was fabricated by electropolymerizing thionine on the surface of ITO electrode. And then glucose oxidase was immobilized on the photoelectrode via the aid of chitosan enwrapping, forming an enzyme-modified photoelectrode. The photoelectrode was employed as an electron acceptor; H2O2 from the catalytic reaction of enzyme was employed as an electron donor, developing an analytical method of glucose without hydrogen peroxidase. In the paper, the pho- toelectrochemical effects of photoelectrode to H202 and glucose were studied. The effects of the bias voltage and the electrolyte pH on the photocurrent were investigated. The linear response of glucose concentrations ranged from 0.05 to 2.00 mmol/L was obtained with a detection limit of 22.0 μmol/L and sensitivity of 73.2 nA/（mmol·L-1）. The applied feasibility of method was acknowledged through monitoring the glucose in practical samples.A new photoelectrochemical method for the determination of glucose based on the photoelectrochemical effect of poly（thionine） photoelectrode to hydrogen peroxide （H202） was reported. The H2Oz-sensitive photoelectrode was fabricated by electropolymerizing thionine on the surface of ITO electrode. And then glucose oxidase was immobilized on the photoelectrode via the aid of chitosan enwrapping, forming an enzyme-modified photoelectrode. The photoelectrode was employed as an electron acceptor; H2O2 from the catalytic reaction of enzyme was employed as an electron donor, developing an analytical method of glucose without hydrogen peroxidase. In the paper, the pho- toelectrochemical effects of photoelectrode to H202 and glucose were studied. The effects of the bias voltage and the electrolyte pH on the photocurrent were investigated. The linear response of glucose concentrations ranged from 0.05 to 2.00 mmol/L was obtained with a detection limit of 22.0 μmol/L and sensitivity of 73.2 nA/（mmol·L-1）. The applied feasibility of method was acknowledged through monitoring the glucose in practical samples.

关 键 词：photoelectrochemical analysis PHOTOELECTRODE GLUCOSE THIONINE 

分 类 号：O482.7[理学—固体物理] Q554[理学—物理]