HPLC-TOF/MS快速鉴别白狼毒药材中的化学成分  

Rapid characterization of chemical compositions in Bailangdu by HPLC-TOF/MS

作　　者：金柔男[1] 何丹 战旗[3] 吕磊[3] 费扬[3] 赵亮[3] 

机构地区：[1]第二军医大学东方肝胆外科医院,上海200438 [2]解放军第一六三中心医院药剂科,长沙410003 [3]第二军医大学东方肝胆外科医院药材科,上海200438

出　　处：《中南药学》2012年第8期571-577,共7页Central South Pharmacy

摘　　要：目的采用高效液相色谱-飞行时间质谱(HPLC-TOF/MS)对大戟科白狼毒2种药材狼毒大戟和月腺大戟中的化学成分进行快速鉴别。方法色谱柱:安捷伦Zorbax Eclipse XDB-C18(3.0mm×50mm,1.8μm),流动相为乙腈-水,梯度洗脱,流速0.4mL·min-1,柱温20℃,紫外检测波长210nm,进样体积5μL;飞行时间质谱采用ESI源,正离子模式监测,质量数扫描范围50～2 000。结果从狼毒大戟提取物中得到23个稳定的色谱峰,月腺大戟提取物中得到26个稳定的色谱峰,并对其所代表的化学成分进行定性分析,发现狼毒大戟和月腺大戟中的化学成分有明显差异。结论采用HPLC-TOF/MS可以快速鉴别大戟科2种狼毒药材中的化学成分,为狼毒药材的质量控制研究及临床合理应用提供依据。Objective To establish rapid HPLC-TOF/MS method to analyze the chemical constituents in Bailangdu including Euphorbia fischeriana Steud and Euphorbia ebiacteolata Hayata.Methods An Agi1ent Zorbax Eclipse XDB-C18 column（3.0 mm×50 mm,1.8 μm） was used for rapid separation and identification of chemical components.The mobile phase consisted of water-acetonitrile in the gradient elution,the flow rate was 0.4 mL·min-1,the column temperature was 25 ℃,the UV detection wavelength was 210 nm and the injection vo1ume was 5 μL.Time-of-flight mass spectrometer（TOF/MS） and electrospray ion source（ESI）were used in qualitative analysis under the positive ion mode,and mass scan range was m/z 50-2 000.Results A total of 26 peaks in the extract of Euphorbia fischeriana Steud and 23 peaks in the extract of Euphorbia ebiacteolata Hayata with good UV absorption and MS response on the chromatogram were selected and identified.The difference in the chemical compositions in the extract of Euphorbia fischeriana Steud and Euphorbia ebiacteolata Hayata was obvious.Conclusion HPLC-TOF/MS method can be used to determine the characterization of the chemical constituents in Bailangdu,which provides an evidence for the quality control and clinical application of Bailangdu.

关 键 词：狼毒大戟 月腺大戟 HPLC-TOF/MS 化学成分 快速鉴别 

分 类 号：R927[医药卫生—药学] TQ460.72[医药卫生—药物分析学]